Question

2. The reaction below is catalyzed by the CK enzyme: NH2 OP H2N H₂N N Ń cooK COO - INN CO + ATP + ADP Below is a picture of the active site for this enzyme. The red atoms are the amino acids in the CK protein. The blue structure is the substrate, the orange is the phosphate end of ATP. All dashed lines are showing interactions and the curved arrows are showing the mechanism. The wavy lines indicate that the atoms shown are part of a larger molecule which is not being shown.

Answer 1

2a) Mg2+ is an intercalating agent.. It will ligated with water molecules and 3 phosphates of ATP and make a complex.

Mg2+ plays a role in neutralizing the negative charges of ATP and stabilizing the phosphoryl transfer reaction

2b) E227 is a residue of ubiquitous mitochondrial creatin kinase enzyme act as a catalytic base to accept the creatin adenine proton.

2c) The ATP binding pocket consists of five highly conserved arginine residues in which one is R315. This R315 arginine residue interact with phosphate oxygen of ATP and help to stabalize the phosphate of ATP.

2d) function of e226 is to form the hydrogen bonding

2e) it is a sequential process because here the product of 1 reaction is the substrate of another reaction.