Homework Answers
1. Transformation is the process of inculcating the vector containing the gene of interest into a bacterial cell by spreading 1microlitre of the inoculum on the LB agar plate in the Laminar Air Flow. The vector inserted in the plasmid cells have a ampicillin resistant gene and galactosidase gene, if the DNA of interest in inserted than it dis orients the open reading frame of the vector and thus the dye X-GAL is not able to express on the medium containing the colonies. Also the E.coli cells carry a massive quality of resistance towards ampicillin.
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.AT&T 12:58 AM 1 0 95% 1:47:09 Exit D5. This week in lab you will observe...
1. Fill in the table above with what you observe on your plates. 2. Bacterial transformation...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
This is what we did in the experiment .First of all,1 µl of plasmid DNA was...
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
This is what we did in the experiment .First of all,1 µl of plasmid DNA was...
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
Need help filling in the chart and answering the questions that go along with it. I...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
1. Fill in the table above with what you observe on your
plates.
2. Bacterial transformation occurred on which agar plate (s)?
What evidence do you have that the bacteria were transformed
here?
3. Which plates have glowing growth? Explain what causes
bacteria to glow.
II. Transformation of E. coli with Plasmid DNA (PGLO) 1. Three LB (Luria Broth) agar plates are obtained. The plates contain: • Plate A: LB-Agar/Ampicillin/Arabinose • Plate B: LB-Agar/Ampicillin • Plate C: LB-Agar 2. Three...
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
This is what we did in the experiment .First of all,1 µl of
plasmid DNA was added into the tube which contains competent cells
and the tube was tapped gently to mix DNA and the competent
bacteria. After that it was placed on ice for 30 minutes. Then, the
tube with the competent bacteria and plasmid were transferred to
heating block at 42 °C and the tube was leaved in there exactly 90
seconds. 0.25 ml of LB broth was...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
We were unable to transcribe this imageTABLE 8-1 Cast of Characters and a Legend of Abbreviations Name Symbol Function in This Experiment Green fluorescent protein GFP It serves as an indicator of successful transformation and gene transcription expression in...
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