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What would you expect to see if GTPgS rather than GTP is added in a reaction...

  1. What would you expect to see if GTPgS rather than GTP is added in a reaction where dynamin subunits are present in a vesicle budding in vitro reconstitution system? (GTPgS is a non hydrolysable analog of GTP)
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Answer #1

The transport of pro-alpha-factor from the
ER to the Golgi apparatus in gently lysed yeast sphero-
plasts is mediated by diffusible vesicles. These trans-
port vesicles contain core-glycosylated pro-alpha-factor
and are physically separable from donor ER and target
Golgi compartment...

ER form of
a-factor (26,000 D) is then converted to the high mo-
lecular weight Golgi form in the presence of ATP, sol-
uble factors and an acceptor membrane fraction;

If GTP replaced by GTPgS then it will inhibit the transport reaction...

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