The transport of pro-alpha-factor from the
ER to the Golgi apparatus in gently lysed yeast sphero-
plasts is mediated by diffusible vesicles. These trans-
port vesicles contain core-glycosylated pro-alpha-factor
and are physically separable from donor ER and target
Golgi compartment...
ER form of
a-factor (26,000 D) is then converted to the high mo-
lecular weight Golgi form in the presence of ATP, sol-
uble factors and an acceptor membrane fraction;
If GTP replaced by GTPgS then it will inhibit the transport reaction...
What would you expect to see if GTPgS rather than GTP is added in a reaction...
1) Describe what would happen with vesicle transport in cells incubated with a non-hydrolysable form of GTP. You get extra points if you describe (correctly) a third one. 2) Tumor cells display a more glycolytic metabon where secrete lactic acid secrete, which in turn acidifies the media. At the same time you do an experiment and you find that these cells decrease their cholesterol uptake. a) describe what that experiment could be. b) how do you explain these results? c)...
To a solution of sucrose, Benedict's reagent is added and the solution is heated. What reaction would you expect and why?
You have discovered a single cellular eukaryotic organism that has very little vesicle trafficking. You suspect that this may be due to the relatively few formed microtubules that are observed in this species. Upon close examination, you find the microtubules that are present are unusually stable. When looking at an amino acid comparison with human alpha and beta tubulin, you notice that the alpha tubulin is identical, but the beta tubulin is different in the region responsible for GTP hydrolysis....
What factors would favor a reaction to be SN1 rather than SN 2? Why it is critical to keep the temperature of the water bath within (+ or -) ½ degree celcius? In which of these solvents would the rate constant for an SN 1 reaction be greatest: 50% water?
2. What product would you expect from the following reaction? 2. What product would you expect from the following reaction? ÇOCH H+ CO CHE A. in ÇOCH3 B CO.CH ÇO,CHE D. CO,CHE E
what products would you expect from the following reaction? (alkene organic reaction with NBS/ CCl4) What product(s) would you expect from the following reaction? You do not have to consider stereochemistry. If more than one structure fits the description, draw them all. Draw one structure per sketcher. Add additional sketchers using the dropdown Separate structures with + signs from the dropdown menu.
D 26. Why do we expect to see more synonymous substitutions than non-synonymous substitutions if a locus is under purifying selection? In your answer, be sure to explain what synonymous vs. non-synonymous substitutions and purifying selection are. Enter answer... D27 In some birds such as phalaropes, males provide all of the parental care to offspring, and females compete with one another for access to males and breeding territory. In this species, would you expect males or females to be larger...
What voltage would you expect to see across the contacts of a contactor that has no voltage applied to its coil but is wired to a live circuit?
Briefly explain what you would expect to see in the UV-visible spectra of the first two compounds you will prepare in this experiment, [Ni(NH3)6][BF4]2 and [Et4N]2[NiCl4]. Which would you expect to give the stronger absorptions, and why?
7. If you actively seek bargains in an efficient market, what would you expect see as the returns on your portfolio, relative to the market, over long periods? (3 points) You should earn a higher risk-adjusted return than the market You should earn the same risk-Cadjusted return as the market before transactions costs a b. c You should earn higher risk-Cadjusted return than the market before d You should earn lower risk-Cadjusted return than the market before transactions costs, but...