Question

You have discovered a single cellular eukaryotic organism that has very little vesicle trafficking. You suspect that this may be due to the relatively few formed microtubules that are observed in this species. Upon close examination, you find the microtubules that are present are unusually stable. When looking at an amino acid comparison with human alpha and beta tubulin, you notice that the alpha tubulin is identical, but the beta tubulin is different in the region responsible for GTP hydrolysis.

You isolated the heterodimer subunits from this new species and put them in a cell free system (no GTP bound heterodimer) with a centrosome nucleation site. How would the dynamics be different from human heterodimer subunits (no GTP bound heterodimer) at the same concentration?

Question 1 options (one or more answers may be correct):

	The new species’ microtubules would treadmill at a maximum concentration of heterodimers, but at a faster rate than human microtubules
	The new species’ microtubules would initiate growth at the same pace as the human microtubules
	The human microtubules would loose more from the (-) end than (+) end compared to the new species
	The new species’ microtubules would grow to the same size as the human microtubules

Answer 1

o Cata 9t oobe a p010 dis a99emble fm the tend 0The neto aperfe9 microtu,bles coould nernal Cella las mfeactobales
Hence option b and d are correct answer​​​​​