Homework Answers
Ans.
#A.
#B.
Add Answer to:
4. Follow the instructions below each of the following figures: O o o oo 000 %...
The following details the results of a serial dilution of E. coli: Effective Dilution Factor on...
The following details the results of a serial dilution of E. coli: Effective Dilution Factor on Plate CFUs 105 1095 106 178 107 5 108 None The "effective dilution factor on plate" already takes into account the fact that you plated 0.1 ml of the dilution. How many CFUs/ml were present in the original sample? a. 1.78 x 10^8 b. 1.14 x 10^8 c. 1.125 x 10^8 d. 1.095 x 10^8
calculate the generation time (minutes/generation) of 4.9*10^6 CFUs/ml calculate the generation time (minutes/ generation) of 155*10^8...
calculate the generation time (minutes/generation) of
4.9*10^6 CFUs/ml
calculate the generation time (minutes/ generation) of
155*10^8 CFUs/ml
for 3hrs 4.9*10^6
for 6hrs155*10^8
The students UR S pes at diferent times during the growth to do dilution plating and get viable plate counts. Here are the results for the samples taken at hours 3 and 6: 3 hours 10 dilution - too many to count 10"dilution - 476 CFUS 10% dilution - 49 CFUS 10 dilution = 5 CFUS 6 hours...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations. b) If you plated a100μL of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
14. Analyze the following serial dilution experiment and answer the questions, each (a and b) worth...
14. Analyze the following serial dilution experiment and answer the questions, each (a and b) worth 10 pts. Given: One ml of the original bacterial culture (tube 0) was added to experiment tube 1, containing 9 ml of media. 7 more experiment tubes were serially diluted in order, the same way, for a total of 8. Each originally contained 9 ml of media, and each received 1 ml from the tube number preceeding it. Two drops from tube #8 were...
Please show how to do these enumeration questions by showing the work. What is the dilution...
Please show how to do these enumeration questions by showing the work. What is the dilution factor if you add 625 µL of culture to 4.375 mL of dilution medium? You count the 10-3 dilution of a yeast suspension using the 1/25 mm2 boxes : you find 45 cells in box 1, 15 cells in box 2, 42 cells in box 3, 23 cells in box 4, and 26 cells in box 5. a) What is the original concentration of...
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1....
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
Now knowing the MPN value, you decide to do a dilution series in order to figure...
Now knowing the MPN value, you decide to do a dilution series in order to figure out the original bacterial concentration of the Swimming Hole, just in case it is not a coliform causing the gastric distress and simply a bacterial overgrowth. You perform the following dilution set: from your 16.3-liter Swimming Hole sample you transfer 10.7 x 10-3 liters into container A which has a volume of 6,250ul, you then transfer 1.7 mL from container A into container B...
1. Vibrio bacteria are abundant in ocean and aquatic environments. Suppose you wish to isolate Vibrio-...
1. Vibrio bacteria are abundant in ocean and aquatic environments. Suppose you wish to isolate Vibrio- specific bacteriophages from seawater. You enrich your sample by spiking the seawater with growth medium and Vibrio fischer. You then isolate your phage and plate it on lawns of bacterial growth to enumerate how many PFUs/mL are in your sample. a. You set up a series of 10-fold phage dilutions out to a TDF of 10', and you plate each of those dilutions mixed...
calculate the generation time (minutes/generation) of
4.9*10^6 CFUs/ml
calculate the generation time (minutes/ generation) of
155*10^8 CFUs/ml
for 3hrs 4.9*10^6
for 6hrs155*10^8
The students UR S pes at diferent times during the growth to do dilution plating and get viable plate counts. Here are the results for the samples taken at hours 3 and 6: 3 hours 10 dilution - too many to count 10"dilution - 476 CFUS 10% dilution - 49 CFUS 10 dilution = 5 CFUS 6 hours...
14. Analyze the following serial dilution experiment and answer the questions, each (a and b) worth 10 pts. Given: One ml of the original bacterial culture (tube 0) was added to experiment tube 1, containing 9 ml of media. 7 more experiment tubes were serially diluted in order, the same way, for a total of 8. Each originally contained 9 ml of media, and each received 1 ml from the tube number preceeding it. Two drops from tube #8 were...
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
Now knowing the MPN value, you decide to do a dilution series in order to figure out the original bacterial concentration of the Swimming Hole, just in case it is not a coliform causing the gastric distress and simply a bacterial overgrowth. You perform the following dilution set: from your 16.3-liter Swimming Hole sample you transfer 10.7 x 10-3 liters into container A which has a volume of 6,250ul, you then transfer 1.7 mL from container A into container B...
1. Vibrio bacteria are abundant in ocean and aquatic environments. Suppose you wish to isolate Vibrio- specific bacteriophages from seawater. You enrich your sample by spiking the seawater with growth medium and Vibrio fischer. You then isolate your phage and plate it on lawns of bacterial growth to enumerate how many PFUs/mL are in your sample. a. You set up a series of 10-fold phage dilutions out to a TDF of 10', and you plate each of those dilutions mixed...
Most questions answered within 3 hours.
-
The rise of online shopping and Amazon, in particular, has
created major dilemmas for large food/CPG...
asked 26 seconds from now -
Assuming that the wave speed varies little when sound waves are
traveling through a material that...
asked 16 seconds ago -
Is oxygen difluoride polar or nonpolar?
nonpolar
polar
ionic
none of the above
asked 11 minutes ago -
Starting from rest, a car accelerates at a constant rate of 2.6
m/s2 for a time...
asked 7 minutes ago -
A pharmaceutical company wants to answer the question whether
time for a drug in pill form...
asked 10 minutes ago -
) Assume that in the market for widgets, demand is highly
elastic compared to supply. If...
asked 20 minutes ago -
Why is a personal interview the most important step in the sales
selection process? What are...
asked 30 minutes ago -
In Python rOverlap (x1, y1, w1, h1, x2, y2, w2, h2) A rectangle
is axis-aligned if...
asked 30 minutes ago -
If we observe in a protein solution that DNA is causing a
mixture 260/280 ratio ~0.9,...
asked 41 minutes ago -
Explain the steps involved in making cDNA from mRNA, and how you
would create a cDNA...
asked 41 minutes ago -
A beta (β) particle is described by which of the following?
Select the correct answer below:...
asked 44 minutes ago -
A new machine costs $150,000, lasts 10 years, has an annual
O&M cost of $50,000, and...
asked 47 minutes ago