a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations.
b) If you plated a100μL of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect to see on a plate? Show and explain your calculations.
Homework Answers
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a) During the exponential phase of bacterial growth, the lab
strain of E. coli has doubling...
a. Write down a differential equation for bacterial growth during the exponential phase (batch culture), where...
a. Write down a differential equation for bacterial growth during the exponential phase (batch culture), where k is the instantaneous growth rate. b. Solve the equation. Your solution should be a continuous function that gives the number of cells as a function of time. c. Write an equation for the doubling time (generation time) in terms of k. d. Parameterize your equation as follows: Assume that you start with 10 cells, and that it takes each cell 30 minutes to...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate 5th plate 6th plate 75 Which plate has the countable number of colonies? What is the dilution factor that produced that plate? • What is the correction factor for the volume? How many cfu/ml are in the culture, based on your calculations? 4. Suppose you plated 1 ml of a 10-7 dilution (tenfold dilutions) of a bacterial broth culture
You inoculate a strain of E. coli into a broth culture and allow it to grow....
You
inoculate a strain of E. coli into a broth culture and allow it to
grow. The broth medium contains three sugars: glucose, lactose, and
maltose. The strain you are growing prefers lactose to maltose. You
measured the growth by doing a viable cell count and drew a growth
curve after calculating the number of CFU/ml over time. There was
an initial lag phase, followed by an exponential phase. There was a
second lag phase of approximately 30 minutes followed...
You inoculate a strain of E. coli into a broth culture and allow it to grow....
You inoculate a strain of E. coli into a broth culture and allow it to grow. The broth medium contains three sugars: glucose, lactose, and maltose. The strain you are growing prefers lactose to maltose. You measured the growth by doing a viable cell count and drew a growth curve after calculating the number of CFU/ml over time. There was an initial lag phase, followed by an exponential phase. There was a second lag phase of approximately 30 minutes followed...
E. Coli has a doubling time of 20 minutes under certain growth conditions. Compute the biomass...
E. Coli has a doubling time of 20 minutes under certain growth conditions. Compute the biomass of E. coli (in pounds) that will accumulate when a single cell of E. coli grows exponentially for 50 hours in these conditions, assuming that nutrients are not limiting. Show your work. (An E. Coli cell has a mass of 10-12gram/cell).
Consider a culture of E. coli growing in exponential phase with a generation time of 20...
Consider a culture of E. coli growing in exponential phase with a generation time of 20 minutes. Beginning with 2 x 106 cells per ml, how long will it take the culture to reach a density of about 3.2 x 107 cells per ml?
The adaptation phase for the E. coli culture lasted A culture of E. coli was grown...
The adaptation phase for the E. coli culture lasted
A culture of E. coli was grown on suitable culture media; aliquots of the culture were serially diluted and spread plated aseptically on agar plate media at the times indicated in the table below. Upon overnight incubation at 37°C, colonies appeared and viable counts were colleted and displayed below. Hours 0 Viable Counts (CFU/mL) 2 .00 x 107 2.10 x 107 2.30 x 107 3.25 x 107 6.60 x 107 1.40...
this is a bacterial growth curve experiment . please explain rhe results Procedure: You will follow...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
your lab manual for instructions, Sample plated Number of colonies on plate TMTC 430 4th plate 5th plate 6th plate 75 Which plate has the countable number of colonies? What is the dilution factor that produced that plate? • What is the correction factor for the volume? How many cfu/ml are in the culture, based on your calculations? 4. Suppose you plated 1 ml of a 10-7 dilution (tenfold dilutions) of a bacterial broth culture
You
inoculate a strain of E. coli into a broth culture and allow it to
grow. The broth medium contains three sugars: glucose, lactose, and
maltose. The strain you are growing prefers lactose to maltose. You
measured the growth by doing a viable cell count and drew a growth
curve after calculating the number of CFU/ml over time. There was
an initial lag phase, followed by an exponential phase. There was a
second lag phase of approximately 30 minutes followed...
You inoculate a strain of E. coli into a broth culture and allow it to grow. The broth medium contains three sugars: glucose, lactose, and maltose. The strain you are growing prefers lactose to maltose. You measured the growth by doing a viable cell count and drew a growth curve after calculating the number of CFU/ml over time. There was an initial lag phase, followed by an exponential phase. There was a second lag phase of approximately 30 minutes followed...
The adaptation phase for the E. coli culture lasted
A culture of E. coli was grown on suitable culture media; aliquots of the culture were serially diluted and spread plated aseptically on agar plate media at the times indicated in the table below. Upon overnight incubation at 37°C, colonies appeared and viable counts were colleted and displayed below. Hours 0 Viable Counts (CFU/mL) 2 .00 x 107 2.10 x 107 2.30 x 107 3.25 x 107 6.60 x 107 1.40...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
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