Question

Need help with 27.46: According to its amino acid composition (I've provided the figure the question is referring too) lysozyme has an isoelectric point that is? Greater than 6, Less than 6, or about 6 are the possible answers.

9:58 ビ山 OBLEMS resulting derivative is treated with Fmoc-Val and DICHOBt, then with trifluoroacetic acid. (b) The cesium salt of Fmoc-Leu is allowed to react with the chloromethyl resin. The resulting derivative is then treated with Fmoc-Val and DIC HOBt, then with tri- fluoroacetic acid. 27.46 According to its amino acid composition (Fig. 27.6, p. 1410), lysozyme has an isoelectric point that is (choose one and explain): (1)<6 (2)about 6 (3) >>6 27.47 Consider the following two peptides, which may have been isolated from the floor of a Big Ten basketball arena circa 2000: B: K-NI-G-H-T A: KE-A-D-Y (a) Which peptide is more basic (has the higher isoelectric point)? Explain. (b) Which peptide will emerge first from an anion- exchange column at pH 6.0 Which one of the following nucleotide sequences (3-end on the left) could be the DNA sequence that codes for the biosynthesis of peptide A? Explain your reasoning. Sequence 1: CGUGAAGCCGACUACUAA (c) Dashboard Calendar To Do Notifications Inbox

Answer 1

27.46. Option 3 is correct.

Lysozyme = Muramidase = N-acetylmuramide glycanhydrolase
It is an antimicrobial enzyme produced by animals as a part of their innate immune response.
It catalyzes the hydrolysis of β-1,4-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan (Cell wall component of bacteria).
Lysozyme is active in a broad pH range (6 to 9).
The isoelectric point of Lysozyme = 11.35 (Greater than 6)

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