Question

11 Department of Biological Sciences BIOCHEMISTRY Test 2.2018 H. Nucleic Acid, Sequencing of DNA: Amino Acids Hydrolysis of salt Laboratory buffers, Polyprotie acids QUESTIONS 1. The DNA strand complementary to the strand 3-GTAGCGTAT-Y' would have the sequence a SLTACOCATAT-3 b.3.CATOXICATA-S c. 3-ATGCGTATA-S" ATATGCGTAS 2. When cytosine is treated with bisulfite, the amino group is replaced with a carbonyl group. Identify the resulting base a. adenine b. guanine c uracil d. thyminee. typoxanthine 3. How many amino acids would be in the protein encoded by a gene with the sequence 3-TACAGCCOGGATTCA-S": a. 5 b. 2 c. 4 d.6 e. 12 4. If a tRNA molecule specialized for transfer of the amino acid ARGININE has the anticodon 3' UCUS, with what codon on mRNA will it bybridize?: a. 5 CAG 3' b. S' GUC 3' c. 5 GTC 3' d. 3 AGA S'e. 5 GAC 3 5. The enzymes that break down foreign, as opposed to native, DNA are called: a helicases b. depolymerases c. restriction endonucleases d. ligases e topoisomerases ceplor 6. Enzyme that transfers a phosphoryl group from a nucleoside triphosphate to an molecule is called: alipase b. DNA polymerase e. phosphatase d. hydrolase e. kinase

Answer 1

1) the DNA strand complementary to 5'-GTAGCGTAT-3' is 3'-CATCGCATA-5'. SO the correct option is (b).

2) Cytosine when deaminated forms uracil

3)3' TAC AGC CCG GAT TCA 5' will have 5 amino acids.

4)3' UCU 5' will hybridise with 3' AGA 5'

5)restriction endonuclease breaks down the foreign DNA into fragments.

6)Kinase transfers phosphoric group from a triphosphate to an acceptor.