Calculate the pH of a solution of proline in which the concentrationof the isoelectric structure is...
Calculate the pH of a solution of proline in which the concentrationof the isoelectric structure is 6 times smaller than that of the species with a net charge of -1. (Hint: use the Henderson-Hasselbach equation and see examples from written info on slides.)
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Calculate the pH of a solution of proline in which the
concentrationof the isoelectric structure is...
1. Calculate the charge on the side chain of the amino acid histidine using the Henderson-Hasselbach...
1. Calculate the charge on the side chain of the amino acid histidine using the Henderson-Hasselbach equation pH = pKa + log (A/HA+). Use pH =7 and a pKa = 6 and solve to 2 significant figures. 2. Cysteine proteases have unusually reactive cysteine side chains. For one of these enzymes in solution at pH 7 you are able to measure the amount of the deprotonated sulfhydryl (ie, the negatively charged species) as 40% of the total. Calculate the pKa...
Gel electrophoresis. Polypeptides can be separated by electrophoresis according to their relative content of acidic and...
Gel electrophoresis. Polypeptides can be separated by electrophoresis according to their relative content of acidic and basic residues. The isoelectric point (pI) of a polypeptide is the pH at which its net charge is zero. (See Fig 3.11 of your textbook). A) At physiological pH (= 7.4), what is the net charge on the tripeptide Asp-Arg-Glu-His? B) Calculate the pI of this tetrapeptide. [Hint: Using the pK, values in Table 2.1 and the Henderson-Hasselbalch equation, determine the pH when the...
A 25.0 mL of a weak acid is titrated with a strong base (0.1 M). Calculate...
A 25.0 mL of a weak acid is titrated with a strong base (0.1 M). Calculate the pH of the solution during the titration if the weak acid concentration is 0.10 M and its Ka = 1.8 x 10-5 and 10.0 mL of base has been added. (Hint: use Henderson-Hasselbach equation). Question options: a) pH= 7.00 b) pH= 5.28 c) pH = 4.56 d) pH= 4.74
1. Calculate the pH of a buffer solution which is 0.50M in NH3 and 0.30M in...
1. Calculate the pH of a buffer solution which is 0.50M in NH3 and 0.30M in NH4Cl. Given Kb = 1.8 x 10^-5 for NH3. a) Calculate Ka. b) Calculate pKa. c) Calculate pH using the Henderson-Hasselbalch equation.
* 2. Calculate the pH of a buffer solution that is 0.050 M in benzoic acid...
* 2. Calculate the pH of a buffer solution that is 0.050 M in benzoic acid (HC,H,O,) and 0.150 M in sodium benzoate (NaC,H,O,). For benzoic acid, K = 6.5 X10. Use the Henderson-Hasselbalch approach. (6 points) Equation: HC,H,02(aq) + H20(1) = H,0*(aq) + C,H,O, (aq) Hint: pH = pKa + log base] (acid]
Calculate pH of a weak acid/conjugate base buffer solution. 1. a) Calculate the pH of 650....
Calculate pH of a weak acid/conjugate base buffer solution. 1. a) Calculate the pH of 650. mL of a 0.211-M solution of acetic acid before and after the addition of 0.123 mol of potassium acetate. pH befor addition = pH after addition = 1 b) Calculate pH of a weak base/conjugate acid buffer solution. A 0.190-M aqueous solution of C2H5NH2 (ethylamine) has a pH of 11.9. Calculate the pH of a buffer solution that is 0.190 M in C2H5NH2 and...
Use the Henderson-Hasselbalch equation to calculate the pH of each solution: A solution that is 0.14...
Use the Henderson-Hasselbalch equation to calculate the pH of each solution: A solution that is 0.14 M in NH3 and 0.20 M in NH4Cl. (Kb= 1.76x10^-5)
Use the Henderson-Hasselbalch equation to calculate the pH of each solution: A. a solution that is...
Use the Henderson-Hasselbalch equation to calculate the pH of each solution: A. a solution that is 0.14 M in HCHO2 and 0.13 M in NaCHO2 B. a solution that is 0.15 M in NH3 and 0.18 M in NH4Cl
Use the Henderson-Hasselbalch equation to calculate the pH of each solution: A. a solution that is...
Use the Henderson-Hasselbalch equation to calculate the pH of each solution: A. a solution that is 0.14 M in HCHO2 and 0.13 M in NaCHO2 B. a solution that is 0.15 MM in NH3NH3 and 0.18 MM in NH4Cl
5. (2pts) To calculate the pH of buffer solution we need to use Henderson-Hasselbalch equation The...
5. (2pts) To calculate the pH of buffer solution we need to use Henderson-Hasselbalch equation The generic form of this equation is: 6. (8 points) What is the pH of a solution that contains 25 ml of 0.10 M HF and 25 ml 0.1M NaOH solution? (Ka of HF -6.8 x 104).
Gel electrophoresis. Polypeptides can be separated by electrophoresis according to their relative content of acidic and basic residues. The isoelectric point (pI) of a polypeptide is the pH at which its net charge is zero. (See Fig 3.11 of your textbook). A) At physiological pH (= 7.4), what is the net charge on the tripeptide Asp-Arg-Glu-His? B) Calculate the pI of this tetrapeptide. [Hint: Using the pK, values in Table 2.1 and the Henderson-Hasselbalch equation, determine the pH when the...
* 2. Calculate the pH of a buffer solution that is 0.050 M in benzoic acid (HC,H,O,) and 0.150 M in sodium benzoate (NaC,H,O,). For benzoic acid, K = 6.5 X10. Use the Henderson-Hasselbalch approach. (6 points) Equation: HC,H,02(aq) + H20(1) = H,0*(aq) + C,H,O, (aq) Hint: pH = pKa + log base] (acid]
5. (2pts) To calculate the pH of buffer solution we need to use Henderson-Hasselbalch equation The generic form of this equation is: 6. (8 points) What is the pH of a solution that contains 25 ml of 0.10 M HF and 25 ml 0.1M NaOH solution? (Ka of HF -6.8 x 104).
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