Question

Question Completion Status: THE TOWN CAP WIVE cuvy UE CE POTURU U UR ECCE v CK CONTENIX catalyzes the breakdown of hydrogen peroxide with the production of water and oxygen. Certain dyes will go from a colorless to a colored form upon oxidation, a property that allows detection of peroxidase activity. In the presence of hydrogen peroxide and peroxidase, orthophenylenediamine (OPD) w be changed from a colorless dye to a yellow compound as it is oxidized. peroxidase H2O + OPD (colorless) (yellow) (reduced form) (oxidized form) H202 + OPD-H Using a spectrophotometer, you quantitated the enzyme reaction by measuring the absorbency from the oxidized OPD at 410nm. The more colored OPD produced in a given time period, the faster the enzymatic reaction. The following are the contents of experimental test tubes (Table 1) and the absorbance readings TUBE ml OPD ml BUFFER (PBS) ml HYDROGEN PEROXIDE ENZYME (Potato extract) Absorbency at 410nm 0.000 0.1 1 (blank) 3.3 0.300 3.3 0.500 22 Egge 0.900

Answer 1

Hypothesis - Peroxidase will catalyze the reduction of H2O2 and its activity can be checked by orthophenyldiamine that will turn yellow as it get oxidised in the presence of H2O2 and Peroxidase.

B. Hydrogen peroxide is the substrate for Peroxidase.

C. Tube 1 beacuse it is blank do not contain enzyme and act as control .

D. I) independent variable - enzymatic activity

Dependent variable - Absorbance since it depends on enzymatic activity , the colour produced by reaction will be a measure of enzyme activity.