Discuss the common tests you would carry out for seedborne bacterial and viral diseases to a group of newly hired seed technicians in state seed laboratory where you are a director
Method for detecting seed borne bacteria:
First you examine how the seed look different from the normal one. The following test is done to detect bacteria:
'Growing on': Sow the test seed samples into seedlings under optimal condition for the disease development in glass house or closed environmental chambers. It is mainly used for large number of Xanthomonads and pseudomonads. Isolate the sample in agar media.
Indicator test: The seed sample is sterilized with 2.6% hypochlorite for 15 min and rinsed in sterile water. The sample is further incubated for 18-24h in sterile water. The water suspension is inoculated into the primary node of 10 day old bean seedlings. The appearance of lesions followed systemic necrosis is positive reaction.
Serology test: This method is used for detection of various phytobacteria. The washing of the working seed sample are cultured for 36h under sterile distilled water. The supernatent is tested with antiserum of suspected pathogen.
Detection of viruses:
Dry seed examination: Visual examination reveal abnormalities like disclouration, shriveling, reduced seed rice size, staining and seed coat necrosis.
Growing on test: Seeds are planted in blotter paper, sand, soil, vermiculture or in growth medium. Seedlings are examined at regular intervals for the viruses.
Indicator test: Extract different parts of seeds and seedlings raised from infected seeds on suitable indicator plants. This test has been used to detect BCMV in bean and urd bean, LMV in lettuce, TMV in tomato and tobacco ring spot virus in soybean.
Discuss the common tests you would carry out for seedborne bacterial and viral diseases to a...
You carry out a serial dilution of a bacterial culture, using dilutions of ⅕, ⅕, 1/100, and 1/10. Then you plate 100 µl of the final dilution and the next day observe 200 colonies on your plate. What was the concentration of the original culture? (5 pts)
How would you carry out the following conversion?
How would you carry out the following reactions?
Discuss the differences in Preferred and Common Stock. Give examples of how you would issue the two types in a corporation where you were CEO.
How would you carry out the synthesis below? Show the reagents
needed for your transformation, but do NOT draw the mechanism for
each step.
SHORT ANSWER #29: How would you carry out the synthesis below? Show the reagents needed for each step of your transformation, but do NOT draw the mechanisms for each step. (10 points) NH2 N
How would you carry out the following conversions?
multiple steps would be required. Separate the steps with numbers.
Be specific with your reaction conditions as solvents and catalyst
may be needed.
Chem 227: Worksheet 8 Multi-step Synthesis (8 points) How would you carry out the following conversions? Multiple steps would be required. Separate the steps with numbers. Be specific with your reaction conditions as solvents and catalyst may be needed HCC - CH(CH3)2 B H ,CC= C-CH(CH3)2 H₂Cc dicholz
please be specific, thank you.
mechanism that would lead to the Ex. carry out the halogenation reaction formation of this product:
How
would you carry out the following conversions? Multiple steps would
be required. Separate the steps with numbers. Be specific with your
reaction conditions as solvents and catalyst may be
needed.
Chem 227: Worksheet 8, Multi-step Synthesis (8 points) How would you carry out the following conversions? Multiple steps would be required. Separate the steps with numbers. Be specific with your reaction conditions as solvents and catalyst may be needed. A.OBH,THF H202 J CH Н В HC C CH(CH3)2 Hассс-...
Accidentally crop out NH2 can i see the full mechanism
Show how you would carry out 2 of the following reactions.
please answer completely and clearly. thank you!
SHORT ANSWER #29: How would you carry out the synthesis below? Show the reagents needed for each step of your transformation, but do NOT draw the mechanisms for each step. (10 points) NH2 N SHORT ANSWER #29: How would you carry out the synthesis below? Show the reagents needed for each step of your transformation, but do NOT draw the mechanisms for each step. (10 points) NH2 N