Question

Single stranded DNA (ssDNA) is a product of DNA denaturation. When compared to double stranded DNA (dsDNA) it is much more flexible with its persistence length (lp) estimated to be an order of magnitude lower than that for dsDNA. If a particular dsDNA sequence has a (4-63 nm): a) Estimate at what concentration of NaCl dsDNA flexibility is comparable to that of ssDNA [NaCl]- a) Estimate at what concentration of MgCl2 dsDNA flexibility is comparable to that of SSDNA [MgCl2]-

Answer 1

Answer:

a) Estimate at what concentration of NaCl dsDNA flexibility is compareable to that of ssDNA

[NaCl] = 2 nM

b) Estimate at what concentration of MgCl2 dsDNA flexibility is comparable to that of ssDNA

[MgCl2] = 5 nm

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