Question

You find that your mutated plate contains a lot of white colonies under UV light and, then, you decide to investigate which DNA region is affected by the transposon, resulting in this white-colony, rather than green-colony, phenotype. You hypothesize that the transposon is inserted into the araC region causing the mutation and plan to use restriction digestion to test your hypothesis. What pair of restriction enzymes would logically make sense to utilize? Select the best answer. A plasmid map is shown below 5.0 Kb stE I1 nd I1 O Clal and Kpnl O Clal and BstEll EcORV and BstEll O BamHI and EcORV

Answer 1

Clal and BstEII because you need to delete araC completely to test that there is no recombination of the transposon. If you leave even a small part, it can function an insert.