Which of the following type of cells would be able to grow on the agar containing...
Which of the following type of cells would be able to grow on the agar containing ampicillin?
An E.coli cell that has no plasmid DNA
An E.coli cell that has a circular plasmid pMiniT 2.o without a PCR product
An E.coli cell that has a circular plasmid MiniT 2.o with an inserted PCR product
Scientists have used cloning technique for a few decades by now. Among all the cloning vectors, there are a few common features, including:
They all have replication origin (ori) so that the plasmid can be replicated just like chromosomal DNA
They contain drug-resistant gene, such as the ampicillin-resistant gene.
Both A and B above
Homework Answers
- An E.coli cell that has a circular plasmid pMiniT 2.o without a PCR product..... because the vector contains ampicillin resistant selectable marker.
- Both A and B above
vectors have origin of replication , mcs (multiple cloning site .... selectable marker like ( antibiotic resistant genes....
Hope it helps .... Kind regards
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Which of the following type of cells would be able to grow on
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Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
Need help filling in the chart and answering the questions
that go along with it. I have added the procedure and the
instructions as well as the "results" that are supposed to be used
to fill in the chart. Thank you!
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