Question

Which of the following type of cells would be able to grow on the agar containing...

Which of the following type of cells would be able to grow on the agar containing ampicillin?

An E.coli cell that has no plasmid DNA

An E.coli cell that has a circular plasmid pMiniT 2.o without a PCR product

An E.coli cell that has a circular plasmid MiniT 2.o with an inserted PCR product

Scientists have used cloning technique for a few decades by now. Among all the cloning vectors, there are a few common features, including:

They all have replication origin (ori) so that the plasmid can be replicated just like chromosomal DNA

They contain drug-resistant gene, such as the ampicillin-resistant gene.

Both A and B above

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Answer #1
  • An E.coli cell that has a circular plasmid pMiniT 2.o without a PCR product..... because the vector contains ampicillin resistant selectable marker.
  • Both A and B above

vectors have origin of replication , mcs (multiple cloning site .... selectable marker like ( antibiotic resistant genes....

Hope it helps .... Kind regards

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