Question

12. What media component is required for NS0 cell culture media that is not typically required for CHO cell culture media? (1 pt) a. Insulin b. Lipids c. Albumin d. Phenol red The data below shows a comparison of the CDF (solid lines) and HCF (dotted lines) in the NSO process (average of three replicate batches, error bars are 1 SD). 18 100 Viability 14 S 12 60 50 E 40 10 Viable Cell 6Density 2 4 8 10 12 Elapsed Time (days) 13. When comparing the two processes, how does the CDF affect cell viability at the end of the run, compared to the HCF? (1 pt) a. Improved viability b. Lower viability c. No change 14. When comparing the two processes, how does the CDF affect viable cell density compared to the HCF? (1 pt) a. b. c. Improved viable cell density Lower viable cell density No change

Answer 1

12. what media component is required for NS0 cell structure media that is not typically required for CHO cell structure media.

ans-

13. when comparing the two process, how does the CDF affect cell viability at the end of the run, compared to HCF?
ans- we can see in the graph that CDF compare to HCF is more prominent to improve cell viability although it decreasing with the days but it is more than HCF

so the right option is (a) improve viability

14.  when comparing the two process, how does the CDF affect viablee cell density, compared to HCF?

ans- we can see in the graph that the line which represents the CDF is more higher than HCF with the time so we can say that CDF improve viable cell density compared to HCF.

so the right option is (a) improve cell viable density