Question

Easy question

Given that a DNA fragment law the end (on right): Which one of the following DNA ends is com, Place the following steps in chronological order when creating a tomato genomic DNA library. The available materials are DNA ligase, restriction enzyme, tomato leaves, plasmid DNK to act as vector that has already been digested with the restriction enzyme, competent bacteria, and nutrient agar plates containing ampicillin. You may use a step more than once. Add DNA ligase Transform bacteria Isolate genomic DNA from leaves Mix genomic DNA with plasmid DNA Digest genomic DNA Digest genomic DNA with restriction enzyme Spread bacteria on nutrient agar medium with ampicillin Given the restriction map of bacteriophage lambda DNA and pUC19 DNA, explain expect to identify a colony carrying the 2.1 kb fragment (position 23.1 - position252) in the We generates. Remember we digested both lambda DNA and pUC19 DNA to completion with the combination of Hindi

Answer 1

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