Question

NOT considerthe following transfer of an amino group from Asp to the α-phenylketoacid catalyzed by Enzyme X: aspartic acid + a-phenylketoacid oxaloacetate + phenylal (Asp) Plots of 1/vo versus 1/[Asp] at different concentrations of the a-phenylketoacid gives parallel lines. Is the reaction a Single or Double (ping-pong) Displacement Reaction? Starting with radioactive asp, explain how you would perform an exchange reaction. (List the substances you would place in the test tube.) What results would you expect?
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Answer #1

1. It is a double displacement/ping pong rxn. Ping pong rxn means one or more substrate are released before all substrate bind the enzyme.

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