1) You have decided to make your own DNA loading dye and get the following recipe...

Question

Question

1) You have decided to make your own DNA loading dye and get the following recipe from your advisor to make a 10ml stock solution.

0.05g Orange G (solid; MW=452.4g)

1.5g Ficol (type 400)

1ml 0.5M EDTA (pH 8)

9ml ddH2O (sterile)

What is the final concentration (in mM) of EDTA in your stock solution? Round to nearest whole number and include proper unit.

Answer 1

Answer #1

M1V1 = M2V2

The initial concentration of EDTA is 0.5 M (M1)and volume is 1 ml (V1). Final volume is 10 mL (V2), an final concentration (M2 = ?).

Thus, (0.5) (1) = (M2) (10)

M2 = 0.05 M

So, the concentration of EDTA in final volume is 0.05 M

Answer 2

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