Question

petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you of 50 ml 1xXTAE to make your agarose gel plus 1 liter of IX TAE for gel runnig will need a minimum normally made as a 50X concentrated stock 8. What is the minimum volume of SOX TAE that you will need to make? You determined in the e previous assignment the exact volume of 50X TAE that you will need.I assignnm suggest that The recipe for one liter of 50X TAE is as follows: you make 40 ml of 50X TAE so that you have some extra (just in case you need it). . 242g Tris base .57.1 ml glacial acetic acid 100 ml 0.5 M EDTA pH 8.0 9. Provide a recipe for 40 ml of 50X TAE 10. When you make your agarose gel, you will need to make 50 ml of 1% (w/v) agarose in 1x TAE How much agarose will you require? When adding your DNA samples to the agarose gel. you will need to add loding dye to the samples. Loading dye contains: .glycerol, which helps the samples sink into the sample wells of the gel; and tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis The recipe for 6X loading dye is as follows: 0.25% bromophenol blue (w/v) 0.25% xylene cyanol (w/v) 30% glycerol in water (v/v) * 11. Although you won't make this for today's lab, assume you need 10 ml of 10X loading dye. Using the information above, provide a recipe. (Important tip: Remember that you have already made 30ml of a 50% stock solution of glyceroLI recomm end determining the concentration of glycerol required for 10X loading dye, then figuring out how much of your 50% glycerol stock will be needed to make 20ml of 10X loading dye.)
9,10,12

Answer 1

5ox Stock oluhion (Lits 57.1m Qlacial acehc acid volume uptore issolved nates 70oml deionize looml 5DmM EDTA Cpt. Solubon 9-68q Tois base dissolu«d in wates(.77ml deioniz 2. 26 m Glacial acehc Acid Date) inal veltme p to H イeparahon ot 50mM EDTA CpHg.O Welgh out 9305ams of EDTA clisodium Solt o m) Stock on (Molecu lay Weight372.2glmo) Dissolve in hooml deionized watey adjust the p w th NaoH 50ml of 17 agarse in IX TAE. AgaTose 9 4. agasose mea n 5 4gm05 agarose An 100ml THE