To do electrophoresis, you will need to make a solution called TAE, which is a specific...

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To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.

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Answer 1

Answer #1

To make 1050ml of 1X TAE (1000ml for running and 50 ml for agarose) we will dilute the 50X TAE as follows:

conc. of 50X TAE *volume required = 1X TAE * 1050

therefore volume of 50X TAE required = 1050/50 = 21ml

So 21 ml of 50X TAE will be required and diluted with 1029 ml of distilled water to make it 1X. This will be used as per requirement for agarose and running buffer.

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Answer 2

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