1. How would you calculate the amount of agarose powder you would need to make 35mL of a 2% (wt/vol) solution?
2. How would you calculate the volume of 20X concentrated buffer to make 35 mL of a 1X solution?
1. How would you calculate the amount of agarose powder you would need to make 35mL...
To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.
You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red. For a total gel volume of 75ml, calculate the mass of agarose powder and volume of Gel Red concentrate needed. Show your work to be eligible for partial credit. Info about stocks: Agarose stock is powder Gel Red concentrate is 25,000X liquid
Describe how much agarose powder (g), 10X TBE (ml), water (ml) and 20,000X greenglo (ul) you would need to prepare a 4.3% 50ml agarose gel.
You have 50 mL of insulin that is 20X concentrated. You need to dilute this insulin in buffer to a concentration of 2X in final volume of 2 mL to give to a patient. How would you prepare this solution?
QUESTION 5 How many grams of Tricine (MW = 179) would you need to make 300 mL of a 20 mM solution? QUESTION 6 How many grams of Tricine (MW = 179) would you need to make 50 mL of a 10 UM solution? QUESTION 7 How many grams of magnesium chloride (x6H2O) are contained in 250 mL of a 0.1 M solution? QUESTION 8 How many grams of sucrose (MW 342) are contained in 250 mL of a 0.1...
Question 23: (10 Marks) a) Given the stock concentration and desired final amount, or desired final concentration, of the following PCR components, calculate the volume of each you would add to a 25 μl PCR reaction mixture. b) You want to analyse the resultant PCR product by agarose electrophoresis. Calculate how much agarose you need to use to make up 50 ml of 1.5 % gel. How much of 5x concentrated loading buffer will you add to load the...
please hlep me answer those three questions asap. Please ignore question 7. Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
How much agarose would you need to prepare a 30 mL, 1.5% (w/v) agarose gel?
To detect the DNA band in your gel, you need to add ethidium bromide (EtBr) from a concentrated stock solution (40 ug/ml) to get a final concentration of 5 ug/ml in your agarose solution. Unfortunately, you made your agarose solution (0.8% agarose and 50 ml volume) before adding the EtBr. Thus, when you add he appropriate volume of concentrated EtBr, you will increase the fila volume of agarose solution. a. What volume of the concentrated EtBr must you add to...
Need help please SOLUTION MAKING in water. Calculate the amount of NaCl you need to weigh out. 1 (a). You need 500mL of 15% (w/v) NaCl 1(b) What approximate volume of water would you dissolve the NaCl in? Please give a range of volumes. 1(c). Assuming that adding the NaCI to your original volume of water caused the volume to increase by 50mL before you added additional water to reach your final volume, what is the DENSITY of your final...