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You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red....

You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red. For a total gel volume of 75ml, calculate the mass of agarose powder and volume of Gel Red concentrate needed. Show your work to be eligible for partial credit.

Info about stocks:
Agarose stock is powder
Gel Red concentrate is 25,000X liquid

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Answer #1

the mass of agarose powder=0.5g/100mL \times75 mL=0.375g

volume of Gel Red concentrate =75mL\times1X gel red/25000X=0.003mL

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