Describe how much agarose powder (g), 10X TBE (ml), water (ml) and 20,000X greenglo (ul) you would need to prepare a 4.3% 50ml agarose gel.
Describe how much agarose powder (g), 10X TBE (ml), water (ml) and 20,000X greenglo (ul) you...
a) Calculate how you would make 1.0 L of 10x TBE (0.9 M Tris, 0.9 M Boric Acid, 20 mM EDTA) Tris MW=121.4 g/mole; Boric Acid MW=61.84 g/mole; EDTA MW=292.2 g/mole b) For your polyacrylamide gel electrophoresis gel you need 50 mL of 0.5x TBE. Calculate and describe how you would make this solution using your stock solution.
How much agarose would you need to prepare a 30 mL, 1.5% (w/v) agarose gel?
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA...
You currently have 20,000X ethidium bromide. You want to make 250 mL of 1X ethidium bromide dissolved in 1X TBE. You currently also have a stock of 10X TBE solution. How much 10X TBE do you need and how much 20,000X ethidium bromide do you need?
1. How would you calculate the amount of agarose powder you would need to make 35mL of a 2% (wt/vol) solution? 2. How would you calculate the volume of 20X concentrated buffer to make 35 mL of a 1X solution?
Lab Homework Convert 875 ul to ml and to I. Each answer should use scientific notation. Show your work. Calculate how you will make the following solutions. You will need to show how much of Y solution you will need and how much water you will need. Show your work: You need to make 125 ml of a 0.3 M sodium acetate (NaAc) solution. Your stock solution is 3 M NaAc. You need to make 750 mL of a 1X...
Agarose does not dissolve well in room temperature buffer. Agarose in its unmelted form is composed of linear polymers of monosaccharides coiled into helical fibers. When melted (at 95°C) these fibers dissociate and can dissolve in water (and the buffer). As the dissolved agar starts to cool the hydrogen bonds re-form, but this time in a 3D mesh of agarose which forms channels the DNA moves through during electrophoresis. The size of these channels depends on the concentration of the...
Can anyone show me step-by-step on how to do the agarose calculations? This week we will run the PCR reactions on an agarose gel and analyze the results. Safety notes: Ethidium bromide is a potent mutagen. Wear gloves when handling containers containing ethidium bromide, when handling gels that have been stained in ethidium bromide, and when working with the computer attached to the gel-doc system. Protocol I. Pouring agarose gels I. Using 10x TAE, prepare 50 ml of 3% (w/v)...
6. When performing agarose gel electrophoresis, how much 6X loading dye should you add to a 8 uL DNA sample before loading it onto the gel? (1 pt)
Thats the entire question. there is notjing else to add. Thanks for your help though. Q3A. You need to analyse a DNA sample on an agarose gel. You added 20 ml of 10X TBE buffer to make a total of 400 ml of running buffer. How will you make 40 ml of a 0.75% agarose gel? 1m Your group needed a much longer time for the samples to migrate as compared to the other groups though you all began electrophoresis...