Question

Agarose does not dissolve well in room temperature buffer. Agarose in its unmelted form is composed of linear polymers of monosaccharides coiled into helical fibers. When melted (at 95°C) these fibers dissociate and can dissolve in water (and the buffer). As the dissolved agar starts to cool the hydrogen bonds re-form, but this time in a 3D mesh of agarose which forms channels the DNA moves through during electrophoresis. The size of these channels depends on the concentration of the agarose. Prepare the gel. In this experiment, our gel is 2% (by weight) agarose. Assuming the final gel will weigh 50 g (by also assuming the approximate density of the gel is that of water 1 g/mL). How much agarose do we need to dissolve in 1xLB buffer (in grams)? grams

Answer 1

..2% means 2 grams in 100 mi, hence if you required to prepare final solution of 50am, you need h 2 g% of 50gm :-) (2/100) 850 = I am agarose. - to prepare 50gm get solution. C As lam Elml) Mathamatically, Di 2g/100m} = 0.02 g/ml in sogm 0.0 2 glux 50g = 1 g/ml) as gaml so, lg² ml (1gm .Agarose