ZuoTi.Pro
Question

Q3A. You need to analyse a DNA sample on an agarose gel. You added 20 ml of 10X TBE buffer to make a total of 400 ml of runni

Thats the entire question. there is notjing else to add. Thanks for your help though.
science   biology   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

1. 0.75% agarose means 0.75 grams in 100 ml

. x grams in 40 ml.

x= 40 *0.75/ 100= 0.30 grams

So 0.30 grams (30 mg) in 40 ml

2. The concentration of the agarose is indirectly proportional to the sieve size. If concentration increases , sieve size decreases which offers more resistance for the DNA to migrate. So the time taken to resolve the DNA on agarose gel will be high.

0 0
Add a comment
Know the answer?
Add Answer to:
Thats the entire question. there is notjing else to add. Thanks for your help though. Q3A....
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • Question 23:                                         &n

    Question 23:                                                                                                  (10 Marks) a) Given the stock concentration and desired final amount, or desired final concentration, of the following PCR components, calculate the volume of each you would add to a 25 μl PCR reaction mixture. b) You want to analyse the resultant PCR product by agarose electrophoresis. Calculate how much agarose you need to use to make up 50 ml of 1.5 % gel. How much of 5x concentrated loading buffer will you add to load the...

  • En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...

    En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....

  • A. Your lab To see if you understand what you did in our lab, answer the...

    A. Your lab To see if you understand what you did in our lab, answer the following based in the procedures for the restriction digest and the gel electrophoresis. 1. Using the PGLO map on p7 of the gel electrophoresis procedure, predict the size of the fragments generated by each enzyme EcoRI Hindill, and Pst! (the sizes you would expect to see on the gel.) (6 pts) Hindill -8 fragments were produced by the restriction enzyme. 2. Answer the calculation...

  • Can anyone show me step-by-step on how to do the agarose calculations? This week we will...

    Can anyone show me step-by-step on how to do the agarose calculations? This week we will run the PCR reactions on an agarose gel and analyze the results. Safety notes: Ethidium bromide is a potent mutagen. Wear gloves when handling containers containing ethidium bromide, when handling gels that have been stained in ethidium bromide, and when working with the computer attached to the gel-doc system. Protocol I. Pouring agarose gels I. Using 10x TAE, prepare 50 ml of 3% (w/v)...

  • Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...

    please hlep me answer those three questions asap. Please ignore question 7. Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...

  • Please correct all questions because i did them wrong MATCHINGEnter the letter that best describes the...

    Please correct all questions because i did them wrong MATCHINGEnter the letter that best describes the reagent in the lefthand box below (12 Points) Answer G2 Buffer Proteinase K C1 Buffer Trypsin PBS VA. disrupts outer cell membrane of 3T3 fibroblasts B. phosphate buffered saline C. used to equilibrate Oiagen Genomic tip column D. precipitates DNA Eendonuclease, double stranded cleavage F. used to wash Qiagen Genomic tip column G. enzyme used to release 313 cells from culture task H. disrupts...

  • help with questions 5 to 10 please PCB 3023L Lab #4 Protocol & Worksheet (30pt) You...

    help with questions 5 to 10 please PCB 3023L Lab #4 Protocol & Worksheet (30pt) You may work in your lab groups durine class. but all written answers must be completed individually in your own words. 1) Using the plasmid map for orientation 1 and the cDNA map as a guide, complete the plasmid map for orientation #2. (4pt) 612 1318 1 - EcoRi EcoRI Xbal ECORV -Xbal- 1662 +Bell EcoRI EcoRV Not FP -- Xhol X 2015 PRSP +...

  • Part I— Just Bad Luck? Brrrring! Brrrring! Jane checked the caller ID on her phone. “Sam!...

    Part I— Just Bad Luck? Brrrring! Brrrring! Jane checked the caller ID on her phone. “Sam! Great!” she thought. It was always nice to get a call from her older brother. But a little twinge of worry tugged at her. It was just a couple of weeks ago that he had mentioned making an appointment with his doctor about some abdominal pain he had been having. “Hi Sam! It’s great to hear from you,” Jane answered. “Hi Jane. Well I...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT