FORMULA : C1V1 = C2V2
C1 = given concentration = 6x
V1 = given volume = ?
C2 = required concentration = 1x
V2 = required volume = 18 ul
6x × V1 = 1x × 18
V1 = 3 ul
FINAL SOLUTION : 3 ul 6x stock + 15 ul diluent
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Q3C.You want to load a total of 18 ul volume to the well. How will you...
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
Before you add your DNA sample to your well, what do you have to add to the sample? O primer O probe O loading buffer (aka loading dye) O size standard (aka marker DNA) Question 10 0.5 pts If you want to estimate the sizes of the DNA fragments in your gel, what do you have to load alongside your DNA samples? primer probe loading buffer (AKA loading dye) size standard (AKA marker DNA)
6. When performing agarose gel electrophoresis, how much 6X loading dye should you add to a 8 uL DNA sample before loading it onto the gel? (1 pt)
1) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. To do a serial dilution of 10% concentration (not volume) for each dilution, how many uL of liquid do you move between each well? ype your answer as a whole number of uL, but do not type the "uL" label. 2) Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. What is the final concentration of well 6 if you...
3. You performed a 20uL (microlitre) PCR reaction last night. How much 6X gel loading dye do you need to add to produce a 1X solution and what will the final volume be after you add the dye?
How to solve for question#6?
5 5. Fill in the table below to set up the reactions for a single and then a set of PCR reactions: Concentration in Volume in 1 Master Mix for four Reagent Stock concentration 10X 25 mM one reaction 1X 2 mM reaction 25 jl reactions Buffer MgClz dNTPs Primer mix DNA template Taq polymerase water Total volume 2.SA 2 al 2.S A Pe 100 nM-0.IA 2 ng/HI 1 unit VS 10 μΜ 0.25 l...
How are we getting the volumes in this solution for example
for 10Xs buffer how did we get 1 microliter please explain for each
reagent how the microliters were obtained; ddH20, 10X buffer A, 1
microgram/microliter DNA stock, and enzyme at 10
U/microliter.
Question: What is the most concentrated stock of buffer A you can make? Now if you want to prepare an enzymatic reaction containing 1X buffer A, 100 ng/ul DNA stock and 1 Unit/ul (U/ul) of enzyme proceed...
"calculate the total mass of DNA that you isolated at the end of Part A" how would you go about doing this, the concentration in my diluted DNA sample was 6.3ug/ml, and my A60 value was 0.126 Abs i don't know if these numbers help.. part A I had to calculate the concentration of DNA in my undiluted sample to which I did Concentration (ug/mL) = (A260 – A280) dilution factor 50ug/mL C=0.126-0.060 20 50nguL= 66ng/uL and go that the concentration...
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA...
Why do we want/need the presence of Mg2+ in a PCR but do not want it present at all other steps of processing/analyzing a DNA sample? (2 marks) Identify and explain what generally occurs in each of the 3 main steps of a polymerase chain reaction. (6 marks) 8. Identify and explain what the 3 different PCR controls inform you about the results of a PCR. (3 marks) Highlight the 2 advantages and 3 disadvantages of using PCR. Ensure to...