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Q3C.You want to load a total of 18 ul volume to the well. How will you mix the above DNA sample with the 6X dye prior to load
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Answer #1

FORMULA : C1V1 = C2V2

C1 = given concentration = 6x

V1 = given volume = ?

C2 = required concentration = 1x

V2 = required volume = 18 ul

6x × V1 = 1x × 18

V1 = 3 ul

FINAL SOLUTION : 3 ul 6x stock + 15 ul diluent

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