3. You performed a 20uL (microlitre) PCR reaction last night. How much 6X gel loading dye...
3. You performed a 20uL (microlitre) PCR reaction last night. How much 6X gel loading dye do you need to add to produce a 1X solution and what will the final volume be after you add the dye?
Homework Answers
The preparation of 1X solution from 6X gel loading dye.
The gel loading dye is 6X times more concentrated than final solution.
The amount of 6X dye to produce 1X solution is calculated as shown below.
Put the values of final volume and initial concentration in the above expression.
Therefore, 3.3 uL of dye is needed to make 1X solution.
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3. You performed a 20uL (microlitre) PCR reaction last night.
How much 6X gel loading dye...
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solve for gel preparation and PCR mastermix calculations with
steps for understanding
TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...
6. When performing agarose gel electrophoresis, how much 6X loading dye should you add to a 8 uL DNA sample before loading it onto the gel? (1 pt)
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
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petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
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solve for gel preparation and PCR mastermix calculations with
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