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6. When performing agarose gel electrophoresis, how much 6X loading dye should you add to a...
3. You performed a 20uL (microlitre) PCR reaction last night. How much 6X gel loading dye...
3. You performed a 20uL (microlitre) PCR reaction last night. How much 6X gel loading dye do you need to add to produce a 1X solution and what will the final volume be after you add the dye?
Can anyone show me step-by-step on how to do the agarose calculations? This week we will...
Can
anyone show me step-by-step on how to do the agarose calculations?
This week we will run the PCR reactions on an agarose gel and analyze the results. Safety notes: Ethidium bromide is a potent mutagen. Wear gloves when handling containers containing ethidium bromide, when handling gels that have been stained in ethidium bromide, and when working with the computer attached to the gel-doc system. Protocol I. Pouring agarose gels I. Using 10x TAE, prepare 50 ml of 3% (w/v)...
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated lab...
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA...
Using Agarose Gel Electrophoresis to identify Hemoglobin Phenotypes 13. Draw the banding patterns you obtained on...
Using Agarose Gel Electrophoresis to identify Hemoglobin Phenotypes 13. Draw the banding patterns you obtained on the space below. Sample 1. A Banding pattern Well 1.0 lbo 2. AS 3. S 4. Unknown 6. O 7. 5 7.0 8. Unknown 8. O 14. Why does sickle cell hemoglobin behave differently from normal hemoglobin during agarose gel electrophoresis? 15. + A child is born with sickle cell disease. What can you conclude about the potential genotypes of the parents? 16. +...
Petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml...
9,10,12
petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
In the nuclease digestion experiment, the scientists use a technique called gel electrophoresis. While this is...
In the nuclease digestion experiment, the scientists use a technique called gel electrophoresis. While this is related to SDS-PAGE used in the separation of membrane proteins, it is NOT THE SAME thing. From the list below, choose all the ways that gel electrophoresis is done for nuclease digestion is different from the gel electrophoresis in SDS-PAGE. Group of answer choices 1.Used to separate DNA or RNA. 2.Used to separate proteins. 3.Prteins and DNA must be separated prior to running the...
You are interested in cloning a gene from the B. sanfranciscus genome, so you design PCR...
You are interested in cloning a gene from the B. sanfranciscus genome, so you design PCR primers that should amplify a 1 kilobase pair (kbp) PCR product that contains the gene of interest. After amplification, you will see if the PCR was successful by loading the entire reaction onto an agarose gel and performing electrophoresis to see if a product of the expected size was generated. To visualize the DNA, you will stain the gel with a fluorescent dye called ethidium bromide, which...
A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis. Assume there is...
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
So far in class I have learned c1v1=c2v2....it is said v1 is usually unknown. I am...
So far in class I have learned c1v1=c2v2....it is said v1 is usually unknown. I am unsure how to solve this problem. 1.You have 23 uL of plasmid DNA that you want to run on a gel, but you only have 7X tracking dye. How much 7X tracking dye would you add to your sample before loading it on the gel?
Before you add your DNA sample to your well, what do you have to add to...
Before you add your DNA sample to your well, what do you have to add to the sample? O primer O probe O loading buffer (aka loading dye) O size standard (aka marker DNA) Question 10 0.5 pts If you want to estimate the sizes of the DNA fragments in your gel, what do you have to load alongside your DNA samples? primer probe loading buffer (AKA loading dye) size standard (AKA marker DNA)
Can
anyone show me step-by-step on how to do the agarose calculations?
This week we will run the PCR reactions on an agarose gel and analyze the results. Safety notes: Ethidium bromide is a potent mutagen. Wear gloves when handling containers containing ethidium bromide, when handling gels that have been stained in ethidium bromide, and when working with the computer attached to the gel-doc system. Protocol I. Pouring agarose gels I. Using 10x TAE, prepare 50 ml of 3% (w/v)...
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA...
Using Agarose Gel Electrophoresis to identify Hemoglobin Phenotypes 13. Draw the banding patterns you obtained on the space below. Sample 1. A Banding pattern Well 1.0 lbo 2. AS 3. S 4. Unknown 6. O 7. 5 7.0 8. Unknown 8. O 14. Why does sickle cell hemoglobin behave differently from normal hemoglobin during agarose gel electrophoresis? 15. + A child is born with sickle cell disease. What can you conclude about the potential genotypes of the parents? 16. +...
9,10,12
petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is no supercoiling.
Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a
explanation for each would be helpful. For the first one I think
its 1000bp but unsure. For the second, I think there is 2 HindIII
sites and for the last one there is 1 EcoRI site? Please
explain.
A plasmid is cleaved...
Before you add your DNA sample to your well, what do you have to add to the sample? O primer O probe O loading buffer (aka loading dye) O size standard (aka marker DNA) Question 10 0.5 pts If you want to estimate the sizes of the DNA fragments in your gel, what do you have to load alongside your DNA samples? primer probe loading buffer (AKA loading dye) size standard (AKA marker DNA)
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