A plasmid is cleaved by restriction endonucleases and analyzed by agarose gel electrophoresis. Assume there is no supercoiling. Answer the following three questions about the plasmid.
Can you please help below? I also don't understand it so a explanation for each would be helpful. For the first one I think its 1000bp but unsure. For the second, I think there is 2 HindIII sites and for the last one there is 1 EcoRI site? Please explain.
Homework Answers
2. The highest fragment is of size 1000bp. When cut by EcoR1, fragment of size 1000bp is found. No other fragment is there. Atleast two fragments should be in the gel, if atleast one site for EcoR1 is there. This means, that the plasmid has not been cut by EcoR1, and is in its supercoiled state in lane1. No site for EcoR1 is there.
The fragment is 1000bp
3. 2 HindIIIsites are there. (3 fragments mean two sites at unequal locations.)
4. 0 sites .
Add Answer to:
A plasmid is cleaved by restriction endonucleases and analyzed
by agarose gel electrophoresis. Assume there is...
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA...
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA with the enzymes BamHI and HindIII separately and then load the fragments into an agarose gel and perform electrophoresis. Next, you perform a Southern analysis using the 4,878-bp EcoRI lambda fragment as a probe. a. Draw a picture of the electrophoresis gel, using the outline of the stained electrophoresis gel in Worksheet 16.IIIB (the two smallest HindIII fragments will run off the gel.) b....
MICROBIOLOGY HELP Exercise: Understanding restriction enzyme digests 1. Below is an image of a plasmid. Various...
MICROBIOLOGY HELP
Exercise: Understanding restriction enzyme digests 1. Below is an image of a plasmid. Various parts of the pl location of two restriction enzyme sites fiction enzyme sites (EcoRI and Hindill) are shown. id. Various parts of the plasmid as well as the ECOR PYFG drugR A researcher performs some restriction enzyme digests of this plasmid. In separate tubes, he digests the plasmid with EcoRI alone. HindIll alone, and EcoRI and Hindill together. He also sets up a sample...
3. The given figure represents the agarose gel electrophoresis results from a restriction digest experiment. Lane...
3. The given figure represents the agarose gel electrophoresis results from a restriction digest experiment. Lane 1 is a DNA ladder (values are in kb) and Lane 2 is the DNA sample cut by a restriction enzyme. What is the size of the band C in lane 2? - 1111111 About 1.8 kb Cannot tell based on the information provided 500 bp About 2 kb © Less than 1.5 bp -
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
please answer question numbe 2. Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Can someone help me with this homework question on gel electrophoresis? Translocation and deletion 3. You...
Can someone help me with this homework question on gel
electrophoresis?
Translocation and deletion 3. You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in determining whether a patient has a mutation within a specific DNA region. You know that this DNA segment of the "normal" gene does not include an EcoRI restriction site; but the mutated DNA segment of the same gene contains an EcoRI restriction site due to a point mutation at...
Can someone please help me answer these? Neat work is greatly appreciated. Explanation will also be...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA with the enzymes BamHI and HindIII separately and then load the fragments into an agarose gel and perform electrophoresis. Next, you perform a Southern analysis using the 4,878-bp EcoRI lambda fragment as a probe. a. Draw a picture of the electrophoresis gel, using the outline of the stained electrophoresis gel in Worksheet 16.IIIB (the two smallest HindIII fragments will run off the gel.) b....
MICROBIOLOGY HELP
Exercise: Understanding restriction enzyme digests 1. Below is an image of a plasmid. Various parts of the pl location of two restriction enzyme sites fiction enzyme sites (EcoRI and Hindill) are shown. id. Various parts of the plasmid as well as the ECOR PYFG drugR A researcher performs some restriction enzyme digests of this plasmid. In separate tubes, he digests the plasmid with EcoRI alone. HindIll alone, and EcoRI and Hindill together. He also sets up a sample...
3. The given figure represents the agarose gel electrophoresis results from a restriction digest experiment. Lane 1 is a DNA ladder (values are in kb) and Lane 2 is the DNA sample cut by a restriction enzyme. What is the size of the band C in lane 2? - 1111111 About 1.8 kb Cannot tell based on the information provided 500 bp About 2 kb © Less than 1.5 bp -
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
III. Subclone the gene into plasmid, extract the plasmid DNA. 5. You know that your insert (gene of interest, GOI) is flanked by the EcoRI sites, which makes this restriction enzyme a perfect candidate to cut out your gene. You also know that the GOI has a unique BamH1 restriction site. After subcloning the PCR product into the plasmid, a purified DNA preparation of the plasmid is digested to completion with BamHI restriction endonuclease. In separate reactions, the same preparation...
please answer question numbe 2.
Restriction endonucleases are bacterial enzymes that recognize, bind, and cut DNA strands at specific recognition sequences. They evolved to protect bacteria from bacterial viruses and are very useful for a wide variety of molecular biology applications. Some of the more common ones include EcoRI which recognizes the 6 bp sequence 5 'GAATTC 3' and cleaves the phosphodiester backbone after the G. Hind III_(AAGCTT), BamHI (G|GATCC), Pstl (CTGCAG), Not! (GC|GGCCGC), Ndel (CATATG) Kpnl (GGTAC^C), Bgl II...
The picture above represents an agarose gel that was used to analyze plasmid DNA after it was cut with the restriction enzyme HindIll. The plasmid was incubated with Hindill until all of the available Hindlll cut sites were cut by HindIll. After running the sample on the gel, three bands were detected (Note that there are three wells shown at the top of the gel for loading samples, however, only the middle well was loaded with sample). Based on this...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Can someone help me with this homework question on gel
electrophoresis?
Translocation and deletion 3. You PCR amplify a 500 bp (base pairs) piece of DNA that has diagnostic value in determining whether a patient has a mutation within a specific DNA region. You know that this DNA segment of the "normal" gene does not include an EcoRI restriction site; but the mutated DNA segment of the same gene contains an EcoRI restriction site due to a point mutation at...
Can someone please help me answer these? Neat work is greatly
appreciated.
Explanation will also be appreciated (optional, if you have
time).
Thank you so much! I will give a big thumbs up :)
(1) You start with the pUC18 plasmid that is a -2.7 kb circular DNA molecule. The multiple cloning site (i.e., polylynker) has unique restriction sites in the following order: HindIII, BamHI, EcoRI. Recall that unique restriction site means that these sites are only found once on...
Most questions answered within 3 hours.
-
Casey is on trial under criminal allegations that she engaged in
fraudulent behavior at the company...
asked 9 minutes ago -
1. A boy stands on one end of a boat, and then walks to the
other...
asked 4 minutes ago -
Surplus Styles is a manufacturer of hair care products,
including shampoos, conditioners, and hair gels. The...
asked 7 minutes ago -
Using an income-expenditure diagram, use the infinite line and
double-drop line tools to show the economy...
asked 15 minutes ago -
Which expression computes a pseudorandom integer between -10 and
10 using rand()
from cstdlib?
A. (rand(...
asked 22 minutes ago -
Roybus, Inc., a manufacturer of flash memory, just reported that
its main production facility in Taiwan...
asked 30 minutes ago -
Two capacitors connected in parallel produce an equivalent
capacitance of 45.0 μF but when connected in...
asked 39 minutes ago -
The differences between the two sets of dependent data are -1,
2,-,2, 2. Round to the...
asked 56 minutes ago -
A χ2-curve, looking at the relationship between age and hours
spent working at an office per...
asked 1 hour ago -
The pH of a sample of water from a river is 5.0. A
sample of effluent from...
asked 2 hours ago -
At the beginning of the period, the Fabricating Department
budgeted direct labor of $136,500 and equipment...
asked 2 hours ago -
Please answer all
____ 28. Rent control is usually
justified on the grounds that it protects...
asked 2 hours ago