So far in class I have learned c1v1=c2v2....it is said v1 is usually unknown. I am...
So far in class I have learned c1v1=c2v2....it is said v1 is usually unknown. I am unsure how to solve this problem.
1.You have 23 uL of plasmid DNA that you want to run on a gel, but you only have 7X tracking dye. How much 7X tracking dye would you add to your sample before loading it on the gel?
Homework Answers
7X loading dye means 1 part of dye and 6 part of sample is required to be loaded in gel i.e. 1microlitre will be dye and 6 microlitre would be the sample
c1v1 formula is required for dilution if 23 microlitre is sample then we use it as
1/6*23 = 4 microlitre of dye will be used
can also be calculated from unitary method
Add Answer to:
So far in class I have learned c1v1=c2v2....it is said v1 is
usually unknown. I am...
This is for an a 3 unknown I am doing in Microbiology. And I don't quite...
This is for an a 3 unknown I am doing in Microbiology. And I don't quite understand it, if you could please help thanks A. How did you prepare the template DNA? B. Name the gene that we will be sequencing in order to identify the unknown. C. Explain why this region is considered to be the target for identification instead of sequencing the entire genome? D. What is the size (in bp) of target DNA in E. coli? B....
I need the answers for questions 2 and 3. My DNA ladder is in lane 2...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
This is also for the Unknown 3 I am doing for microbiology. If you could please...
This is also for the Unknown 3 I am doing for microbiology. If you could please help in detail thanks. A. Now that you have confirmed that PCR product is of correct size, using agarose gel electrophoresis, how will you find out that the amplified DNA fragment is our desired target region? B. What is the principle of Sangers’ sequencing technique? Use a labeled diagram (may copy & paste image from internet) to briefly describe the technique. Write two differences...
Yes everyone who observed the incident should report it, I am usually not a fan of...
Yes everyone who observed the incident should report it, I am usually not a fan of a snitch, but in this situation if you do not report then you are a part of the problem. Not only is this gesture breaking hospital policies and The American Podiatric Medical Association Code of Ethics, but also is breaking the law with no consent from the second party involved. The pediatric resident may have had the same incident occur to her at one...
I am creating a recursive function in c++ and have the following code so far. However,...
I am creating a recursive function in c++ and have the following code so far. However, can you help me fix it so that it doesn't crash when negative numbers are entered. If negative numbers are entered i want to let the user know negative numbers are not accepted and to try again #include <iostream> using namespace std; int multiplication(int x, int y) { int sum = y; //if value of x is 1, then result of x*y will be...
(Below I have posted my full assignment so my question makes sense with context. The part...
(Below I have posted my full assignment so my question makes sense with context. The part I am struggling with is section e. I am not sure how to create the said class. If you could help it would be appreciated. Also, this is all HTML code.) In this assignment you will create a web page about your favorite TV show of all-time and link it to an external style sheet to apply a few CSS rules to it. Follow...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
Here is what i have so far... However i am having a little bit of trouble...
Here is what i have so far... However i am having a little bit of trouble with it. when inputting a number the sum comes out perfect but when i break the digits up i cant do mmore than 4 digits and i cant do a negitive number... someone help. QUESTION: Write a program that prompts the user to input an integer and then outputs both the individual digits of the number and the sum of the digits. For example,...
This is my code so far for the assignment. I am stuck on the last 3...
This is my code so far for the assignment. I am stuck on the last 3 tasks which are task#3, task#4 and task#5. Task #3 String Comparisons In checkForDiscount() method of the driver: 1. Figure out if there is a discount of $2 on this order, due to the customer's name being either Mike or Diane 2. Return either 2 or 0 as the discount to the cost of the pizza 3. Compile, debug, and run. You should now be...
PHP Programming Question. Use the techniques you learned so far to create an Address Book application...
PHP Programming Question. Use the techniques you learned so far to create an Address Book application that stores names, e-mail addresses, and phone numbers in a text file. Validate all input fields and include functionality that allows the user to view the address book. Also, include code that sorts the address book by name and deletes duplicate entries. Each page in the application should have a link back to the main page. Be creative and add extra features if you...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
Most questions answered within 3 hours.
-
Two capacitors connected in parallel produce an equivalent
capacitance of 45.0 μF but when connected in...
asked 1 minute ago -
The differences between the two sets of dependent data are -1,
2,-,2, 2. Round to the...
asked 17 minutes ago -
A χ2-curve, looking at the relationship between age and hours
spent working at an office per...
asked 49 minutes ago -
The pH of a sample of water from a river is 5.0. A
sample of effluent from...
asked 1 hour ago -
At the beginning of the period, the Fabricating Department
budgeted direct labor of $136,500 and equipment...
asked 2 hours ago -
Please answer all
____ 28. Rent control is usually
justified on the grounds that it protects...
asked 2 hours ago -
PARTS A-D HAVE BEEN ANSWERED. WAS TOLD TO REPOST. ONLY ANSWER
PARTS E and F.
A...
asked 2 hours ago -
2) You are given the task of finding a representation for a
circle in a drawing...
asked 3 hours ago -
STUDY QUESTION: Does use of diet drug fen-phen
(fenfluramine-phentermine) cause valvular heart disease?
HINT: Valvular heart...
asked 3 hours ago -
1. An object weighing 40 N rests on a surface. The coefficient
of friction is 0.35....
asked 4 hours ago -
Investor company owns 35% of investee company voting stock and
accounts for the investment under the...
asked 5 hours ago -
The number of major faults on a randomly chosen 1 km stretch of
highway has a...
asked 6 hours ago