Question

Lab Homework Convert 875 ul to ml and to I. Each answer should use scientific notation. Show your work. Calculate how you will make the following solutions. You will need to show how much of Y solution you will need and how much water you will need. Show your work: You need to make 125 ml of a 0.3 M sodium acetate (NaAc) solution. Your stock solution is 3 M NaAc. You need to make 750 mL of a 1X TBE solution. Your stock solution is 10X TBE. You need to make a 25 mL solution of 0.5 ug/ml ethidium bromide. Your stock solution is 10 mg/ml. (HINT: The units are different).

Answer 1

1. 875 ul = 0.875ml = 0.000875 l

(1ml = 1000ul

1L =1000ml

1 L= 1000000ul)

2.0

1. we have given 3M NaAc stock solution we have to make 125ml of 0.3M solution

We know the formula C1V1=V2V2( where C1= initial concentration , V1= initial volume, C2= final concentration , V2- final volume.

C1= 3 ,V1=? C2= 0.3 V2= 125

C1V1=C2V2

V1=C2V2/C1

V1= 0.3 x 125 /3

V1= 12.5

Take 12.5ml of 3M stock solution and mix with 112.5 ml distilled water

2. we have given 10x TBE stock solution we have to make 750 ml of 1x solution

We know the formula C1V1=V2V2( where C1= initial concentration , V1= initial volume, C2= final concentration , V2- final volume.

C1= 10 ,V1=? C2= 1, V2= 750

C1V1=C2V2

V1=C2V2/C1

V1= 1 x 750 /10

V1= 750/10 = 75

Take 75 ml from 10x stock and mix with 675 ml of distilled water.

3. we have given 10mg/ml= 10000ug/ml stock solution we have to make 25ml of 0.5ug/ml ethidium bromide solution

We know the formula C1V1=V2V2( where C1= initial concentration , V1= initial volume, C2= final concentration , V2- final volume.

C1= 10000ug/ml ,V1=? C2= 0.5 V2= 25

C1V1=C2V2

V1=C2V2/C1

V1= 0.5 x 25/ 10000

V1= 12.5/10000

=0.00125

Take 0.00125 ml = 1.25ul of stock and mix with 24.99875ml of distilled water/buffer.