You have a 50X stock of electrophoresis buffer and you need 150ul of 1X. How do...
You have a 50X stock of electrophoresis buffer and you need 150ul of 1X. How do you make this happen?
For Vs i got 3ul of 50X stock needed to prepare 150 ul of 1X.
Then I got 147ul of h2o needed to prep 150ul of 1X.
I am wondering if these are correct and if I am using the right wording with my answers? Should i be wording them in a different way? just don't want to get marked down for silly points when my math is correct.
Homework Answers
STEPS WILL BE LIKE THIS:
Since we have 50 X and need to prepare the 150ul of 1 X so we can use the formula:
M1 x V1 = M2 x V2
50 X x V1 = 1 X x 150ul
Hence V1 = 3 ul
Therefore to prepare 150ul of 1 X ; we need to take 3 ul of 5 X and dilute it to 150ul means add 147 ul water.
Add Answer to:
You have a 50X stock of electrophoresis buffer and you need
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I need the answers for questions 2 and 3. My DNA ladder is in lane 2...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
you dont need numbers , just budgets that they are going to need to make Think...
you
dont need numbers , just budgets that they are going to need to
make
Think about your personal budget. How different do you think it is from a company budget? Understanding the different components outlined in a company budget, in addition to the order of completion, helps a business owner, professional, and/or student set reasonable and attainable goals. Budgeting is considered a benchmark document that is pertinent to setting goals and answering questions. Read the following scenario and complete...
LANDON: Do you have 10 or 15 minutes that you can spare? YOU: Sure, I've got...
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Think about your personal budget. How different do you think it is from a company budget? Understanding the diff...
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To do electrophoresis, you will need to make a solution called TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. Assuming no errors and no spills, you will need a minimum of 50 ml 1X TAE to make your agarose gel plus 1 liter of 1X TAE for gel running buffer. TAE is normally made as a 50X concentrated stock.
lab math about micropipetting
Pipette Calibration Check Use this link below to view short video that demonstrates pipette calibration check. https://www.youtube.com/watch?v=1zYxCsKgs80 Lab Math Review 1. Calculate the Mean delivered volume of the P200 pipette being checked using the values(L): 150, 151, 151, 150 and 152. So.8 2. Dilute a 100X (100 times concentrated) stock solution with D-H2O to make 5L of a 1X solution. What volume of stock and D-H20 are needed? 3. A solution contains 10g of solute dissolved...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
you
dont need numbers , just budgets that they are going to need to
make
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LANDON: Do you have 10 or 15 minutes that you can spare? YOU: Sure, I've got a meeting in an hour, but I don't want to start something new and then be interrupted by the meeting, so how can I help? LANDON: I've been reviewing the company's financial statements and looking for general ways to improve our performance, in general, and the company's return on equity, or ROE, in particular. Amelia, my new team leader, suggested that I start by...
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