1. How much of a Na2Co3 stock solution do you need to make 150 ml of...
1. How much of a Na2Co3 stock solution do you need to make 150 ml of a 5 % Na2Co3 working solution ( mw=105.99g/mole)
2. You purchased a t7 DNA ligase , a 10x buffer [50mM tris ph 7.5, 100mM Na2co3, 70mM ATP, 250ug/ml bovine serum Albunium ] is supplied with it. what is the final Molarity of Tris and Bovine Serum Albumin when you use the ligase enzyme to perform a ligand reaction? I know the answer to it its a factor of 10 but still not grasping the concept.
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1. How much of a Na2Co3 stock solution do you need to make 150
ml of...
k. How would you prepare a solution of 250 ml SN buffer (0.1M NaCl, 50mM EDTA,...
k. How would you prepare a solution of 250 ml SN buffer (0.1M NaCl, 50mM EDTA, 1mM Tris) if given NaCl solid (gram molecular weight = 58.44), a 5M stock EDTA, and a 100mm stock Tris?
Question 6 Using stock solutions to make up a solution: from final concentration to volume You...
Question 6 Using stock solutions to make up a solution: from final concentration to volume You need to digest a sample of DNA with a restriction enzyme. You will do this reaction later in the semester. The buffer for this reaction has a final concentration of 40 mM Tris, pH 7.5, 10 mM MgCl2 and 50 mM KCl. You have the following stock solutions: 0.5 M Tris, pH 7.5, 1 M MgCl2 and 2 M KCl. How would you make...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution...
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
1- a- How do you prepare 600 mL 4 N H2SO4 solution using 50% Sulfuric acid...
1- a- How do you prepare 600 mL 4 N H2SO4 solution using 50% Sulfuric acid stock solution (d: 1.403 g/mL MW:98g/mole)? b- If you took 10 mL from this sulfuric acid solution (4N) and diluted to 1000 mL, what would be the % (w/v) and ppm concentration of the final solution.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Need major help 1. How many milligrams of Ruthenium-tris(2,2'-bipyridyl) dichloride [Ru(bpy)3] do you need to make...
Need major help
1. How many milligrams of Ruthenium-tris(2,2'-bipyridyl) dichloride [Ru(bpy)3] do you need to make one hundred microliters of a 100 mM solution?: 2+ The molecular weight of Ru(bpy)3 is 640.53 grams/mole. N. 2. How many microliters of the 100 mM [Ru(bpy)] solution do you need to make one milliliter of a 10 μM solution? 3. How many samples can we make from this stock solution, assuming one mL each of a 10 uM sample?
How much sucrose do you need to make 50 ml of a 10% solution? Sucrose is...
How much sucrose do you need to make 50 ml of a 10% solution? Sucrose is a solid with a molecular weight of 342.3. Show your calculations, and give complete instructions.
k. How would you prepare a solution of 250 ml SN buffer (0.1M NaCl, 50mM EDTA, 1mM Tris) if given NaCl solid (gram molecular weight = 58.44), a 5M stock EDTA, and a 100mm stock Tris?
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
please hlep me answer those three questions asap.
Please ignore question 7.
Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
Need major help
1. How many milligrams of Ruthenium-tris(2,2'-bipyridyl) dichloride [Ru(bpy)3] do you need to make one hundred microliters of a 100 mM solution?: 2+ The molecular weight of Ru(bpy)3 is 640.53 grams/mole. N. 2. How many microliters of the 100 mM [Ru(bpy)] solution do you need to make one milliliter of a 10 μM solution? 3. How many samples can we make from this stock solution, assuming one mL each of a 10 uM sample?
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