k. How would you prepare a solution of 250 ml SN buffer (0.1M NaCl, 50mM EDTA,...

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k. How would you prepare a solution of 250 ml SN buffer (0.1M NaCl, 50mM EDTA, 1mM Tris) if given NaCl solid (gram molecular

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Answer 1

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250mL of Molanity O•IMNacl mass molarmas x Volume Mas 0.1 mor x 58 59 x 250x10K mol mas Nacl= 1.46259 125ml ef loomM EDTA M,V = M2V Stock diluted 5MXV = 100 mM x 125 mL = loo xoX 125 mL V=12.5m2 125 ml of 2mM

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