Question

Calculate the approximate number of amino acid residues contained within a single Protein X molecule.

Questions #20-23 pertain to the SDS-PAGE images shown below. Gel A (left) is a non-reducing SDS-PAGE. Gel B (right) is a reducing SDS-PAGE. The M lanes contain molecular weight markers. Lanes 1, 2, &3 were loaded with equal volumes of a purified preparation of Protein X. Lane 4 was loaded with an equal volume of a crude preparation of Protein X (prior to purification). M 1 2 3 M 4 150KD- ー → 150KD 25KD B

Answer 1

The average size of aminoacid in a protein is approximately 110 daltons,

and clearly the size of the protein is approx 150k Ddaltons which are equal to 150 000 daltons.

So the number of aminoacids should be approximately 150 000 / 110 = 1363.63 aminoacids.

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