Confused on how to answer
questions E, F, and G.
The answer for E is Cysteine,
F (i) is 2
F (ii) is 2
F (iii) is 180
Homework Answers
E) In lane 8 in SDS-PAGE, the di-sulfide bond (covalent bond) has been broken with the help of SDS , a anaionic detergent. Disulphide bond formed between sulfer containing amino acid which is Cystein. Cystein fromed disulphide bond between them.
F) After reducing the native protein broken into large and small subunits which was held together by the disulphide bonds in lane 8. that's why ..
(i) there is 2 large subunits ( which is 60 Kda and 2 fold thick band in unreduced conditions)
(ii) 2 small ubunits as well from reduced protein we can see that
(iii) Total molecular weight of protein X is 180 ( 60+60 for large subunit and 30+30 for small subunits)
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Confused on how to answer
questions E, F, and G.
The answer for E is Cysteine,...
a-d plz 7. Protein purification. As a graduate student, the first protein I purified was RNA...
a-d plz
7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP) from E. coli- Some physical and chemical properties of Ecoli RNAP Molecular max470,000 g/mol polynentide compasitian (subunits): (50 kDa), k andok a pl = 5.34 substrates: NTPs cofactor: Mg? Purification protocol. E coli cells were broken usine Isozyme, yielding a cellualar extract containing a protcome solution 4M (NHOSO, was added to the cellular extract. A white protein precipitate was formed incuding RNAM...
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with known molecular weights of 10 KDa and 80 KDa. Both of these
proteins consisted of a
single polypeptide chain. This mixture was then separated by gel
filtration (below) or by SDS-PAGE (below).
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How can I do this problem step by step? How did they get the following answers?
How can I do this problem step by step? How did they get the
following answers?
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a-d plz
7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP) from E. coli- Some physical and chemical properties of Ecoli RNAP Molecular max470,000 g/mol polynentide compasitian (subunits): (50 kDa), k andok a pl = 5.34 substrates: NTPs cofactor: Mg? Purification protocol. E coli cells were broken usine Isozyme, yielding a cellualar extract containing a protcome solution 4M (NHOSO, was added to the cellular extract. A white protein precipitate was formed incuding RNAM...
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Two different experiments were performed to determine
the quaternary structure of HCV reverse transcriptase as well as
the molecular size of each subunit. In these experiments, HCV
reverse transcriptase was mixed with equal masses of two proteins
with known molecular weights of 10 KDa and 80 KDa. Both of these
proteins consisted of a
single polypeptide chain. This mixture was then separated by gel
filtration (below) or by SDS-PAGE (below).
Gel Filtration Column: The absorption as a function of the...
How can I do this problem step by step? How did they get the
following answers?
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Please address both of the underlined points in your
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