Homework Answers
The protein has four subunits... out of four two are larges subunits each with 35000 daltons and two are small subunits each with 25000 daltons.
So, the molecular weight of the proteins is
35000*2 = 70000
20000*2 = 40000
Total is =110,000
This weight is equls to size exculsion column.
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a-d plz
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7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP)...
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I am having trouble with both number 4 questions and number 8. • Ο Α Υ...
I
am having trouble with both number 4 questions and number 8.
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4. The molecular weight of an Dalton, as determined by sedimentation equilibrium measurements and by gel filtration chromatography. The SDS-polyacrylamide gel electrophoresis (SDS PAGE) of the protein yields a single band corresponding to molecular weight of 70,000 Dalton. However, in the presence of the reducing agent, B-mercaptoethanol, the SDS PAGE shows two bands, corresponding to molecular weights of 30,000 and 20,000 Dalton. unspecified protein, at physiological conditions, is 70,000 ure a. From these data, describe the native protein in terms...
a-d plz
7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP) from E. coli- Some physical and chemical properties of Ecoli RNAP Molecular max470,000 g/mol polynentide compasitian (subunits): (50 kDa), k andok a pl = 5.34 substrates: NTPs cofactor: Mg? Purification protocol. E coli cells were broken usine Isozyme, yielding a cellualar extract containing a protcome solution 4M (NHOSO, was added to the cellular extract. A white protein precipitate was formed incuding RNAM...
7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP) from E. coli. Some physical and chemical properties of E. coli RNAP Molecular mass = 470,000 g/mol polypeptide composition (subunits): a (50 kDa), B (150 kDa) and 6 (70 kDa) pl = 5.34 substrates: NTPS cofactor: Mg Purification protocol. E. coli cells were broken using lysozyme, yielding a cellualar extract containing a proteome solution. 4M (NH4)2SO4 was added to the cellular extract. A white...
I
am having trouble with both number 4 questions and number 8.
• Ο Α Υ ν Α Α Α Α Clipboard Font Voice Sensitivit blood vessels from forming. All of these actions aid in destroying cancer cells. 4. a. You purified a peptide having molecular weight 2000D. How do you raise the polyclonal antibody against this peptide? b. Why does the polyclonal antibody characterize as heterogeneous? 4. How do you test herpes simplex virus biochemically? 5. Name the type...
PARTB Experiment 1 (201). Molecular Weight Determination The 1-1. You will be in the wild with me is found in rabbit serum. This protein is called serumbumin. The sandard unknown proteins and the rabbit serum prins for this experimentarehe pre- tined to allow you to follow the portion during crophews Background Information A first step in the analysis of a protein in the molecular biology laboratory frequently involves determination of its sie or molecular weight. The molecular weight of a...
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