Question

Question 19 (1 point) Saved Forward Primer: GTG ATG CGG GTT TGT GCA GAC (5' to 3' on coding strand) Make a reverse primer from the following sequence, also from the coding strand, that is GC clamped and the Tm is within plus or minus 1 degree C (using the rule of thumb for calculating Tm we used in class). Please write the primer in its own 5' to 3' sequence, so we can send it out to be synthesized. ATC GCT GTG CTC CAC GAG ACA GTC CGT TGA (5' to 3' on coding strand) The spacing is just to aid in counting. Please write the primer in its own 5 prime to 3 prime sequence, so we can send it out to be synthesized. OCTC CAC GAG ACA GTC CGT TG O GTG CTC CAC GAG ACA GTC CG O ATC GCT GTG CTC CAC GAG ACA O GAG GTG CTC TGT CAG GCA AC O A CAC GAG GTG CTC TGT CAG GC O TAG CGA CAC GAG GTG CTC TGT OTCA ACG GAC TGT CTC GTG GAG OCG GAC TGT CTC GTG GAG CAC AG OTGT CTC GTG GAG CAC AGC GAT

Answer 1

5' TCA ACG GAC TGT CTC GTG GAG 3'

Coding strand sequence is,

5' ATC GCT GTG CTC CAC GAG ACA GTC CGT TGA 3'

Now, the complementary sequence of the coding strand will be the sequence of the primer. So, the complementary sequence of the coding strand is:

3' TAG CGA CAC GAG GTG CTC TGT CAG GCA ACT 5'

Now, if you arrange in 5' to 3' orientation, then the sequence will be,

5' TCA ACG GAC TGT CTC GTG GAG CAC AGC GAT 3'