Question

Lipids from 78.2 grams of seal blubber were extracted with 100 mL of diethyl ether. The solution was dried and the lipids emulsified in 75 mL of aqueous solution that contained 66.7 μmoles of polar bear pancreatic lipase and 33.3 μmoles monoacylglycerol lipase. The emulsion was stirred at 25 °C for 15 hours, after which the lipids were extracted with 75 mL of carbon tetrachloride. Draw the chemical structure of the solute that is expected to be most prevalent in the aqueous phase.

Answer 1

The lipid extracted with diethyl ether (lipid is water insoluble)

the solution is dried and lipids are emulsified in 75mL of aqueous solution with 66.7 μmoles of polar bear pancreatic lipase and 33.3 μmoles monoacylglycerol lipase.

So this combination will hydrolyze the lipid as

lipid + 2 H₂O $\rightleftharpoons$ glycerol + fatty acid anions

The left over lipid was extracted with CCl4.

So in the aqueous phas there will be

monoacylglcyerol and fatty acid