Homework Answers
- Step of ELISA are given below:
- Antibody coating specific capture antibody is immobilized on a high protein binding plate by overnight incubation. Plates are blocked with irreverent protein.
- Next step is protein capture sample dilutions and standard illusions are added to the Wells and will be captured by the bound antibodies.
- Detection antibodies specific biotinylated detected antibodies are added to the well how to enable detection of the captured protein.
- Streptavidin-enzyme conjugate with Alkaline phosphatase or Horseradish peroxidease is added to the Wells and will bind to the biotinylated antibodies.
- Addition of substrate calorimetric substrate is added to the Wells and will form Om a coloured solution when catalyzed by the enzyme.
- Analysis absorbance is measured in an ELISA reader and the amount of protein in the sample is determined.
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Below is a diagram with an ELISA assay. Each number represents a step in the sequence...
2. For the ELISA assay depicted below, what do the arrows indicate? Click here to enter...
2. For the ELISA assay depicted below, what do the arrows indicate? Click here to enter answer 3. What would happen in terms of the colorimetric readout if you forgot to do the wash step after Step D (above)? Click here to enter answer-
Question 12 parts a,b,c Q12. Using the information provided below on ELISA, answer the following questions...
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
5) The diagram below represents the energy diagram for the reaction of 1-butyl chloride with water....
5) The diagram below represents the energy diagram for the reaction of 1-butyl chloride with water. On the diagram, draw and indicate the position of each of the following: (0.9 points) a) structures of reactants, products, and intermediates (indicate the position of each on the diagram) b) indicate the positions of all transition states (label as "TS") c) indicate the activation energy of the rate-determining step (label as Ea) Energy Reaction Coordinate 6) Below is an energy diagram that represents...
Answer please? The sequence below represents a middle section of the template strand of DNA of...
Answer please?
The sequence below represents a middle section of the template strand of DNA of a structural gene in an eukaryote organism. Please fill in the blanks that correspond. The consensus sequences that the spliceosome recognizes are marked in red. The intron(s) are marked in lowercase. YOUR RESPONSES SHOULD ALL BE IN UPPER CASE. Amino acid sequences should be written in the format ALA-TYR-LEU Stop codon is not written. DNA: 3 CATGGACAGgtaagaatacaacacagGTCGGCATGACG 5' What would be the immature RNA...
The diagram below (the one on the right) represents the reaction written above it (on top...
The diagram below (the one on the right) represents the reaction
written above it (on top of the picture). Assign each step with a
reactant, a product or an intermediate. DRAW THE TRANSITION STATE
FOR EACH STEP.Keep in mind that it is easier to neutralize
charges than to create some. Nevermind what is handwritten on the
picture.
NOTE: THERE IS 2 STEPS (2 ARROWS) IN THE REACTION,
therefore there is 2 bumps on the diagram & 2 transition states
(that...
please help me with these questions. thank in advance! The diagram below represents an elevator used...
please help me with these questions. thank in
advance!
The diagram below represents an elevator used to lift concrete blocks to upper levels on a construction site. The belt travels at a velocity of 0.9 m/s and is inclined at 26° above the horizontal plane. Calculate the friction force and the coefficient of static friction between each block and the belt. Each block weights 16.5 kg. А NOT TO SCALE $300 Concrete is used in a variety of applications from...
1. (12 pts) Fill in the boxes over each arrow with the letter that corresponds to the reagents needed to perform each step of the multistep sequence shown below. Reagents may be used more than once,...
1. (12 pts) Fill in the boxes over each arrow with the letter that corresponds to the reagents needed to perform each step of the multistep sequence shown below. Reagents may be used more than once, or not all. Pay close attention to the numbered carbons in the starting material, and be sure that your answer accounts for the position of these carbons in the final product. 1 3 3 3 2 2 MCPBA CF3SO3H A) HOCH3, DCC, DMAP E)...
D. The data below is for the reaction sequence A- Elementary step A, H/kJ mol A-...
D. The data below is for the reaction sequence A- Elementary step A, H/kJ mol A- B B- C C D B- C- E /kJ mol Hint: Use the data to prepare a graph of the reaction profile and then answer the questions below. Your graph should be properly labelled and drawn to scale. Potential Energy Reaction progress (a) Whichof the following steps has the smallest rate constant? AB Bc (b) Which of the following steps has the largest rate...
D) 16.94% Refer to the diagram below for questions 25 and 26: The Chart Below represents...
D) 16.94% Refer to the diagram below for questions 25 and 26: The Chart Below represents the Supply and Demand for Auto Insurance policies for teenagers with bad driving records. Price represents the annual premium paid for the policy to cover the student for a calendar year. The quantity represents the number of policies actually sold by the insurance companies to teenagers who want to drive. Price $1,900 $1,400 # 980- Demand -- puantity 25) 2500 3,800 6,500 If, in...
step by step please Using the circuit diagram below, answer the following questions: Α Ω Β...
step by step please
Using the circuit diagram below, answer the following questions: Α Ω Β Ω 15.0 V (C Ω D Ω ma 15.0 v Assume the following resistor values for the resistors above: A-6 Ohms B-6 Ohms C-8 Ohms D-4 Ohms a) What is the value of l at resistor AQ? (12 points) b) What is the potential difference across the Ba? (4 points) What is the power at the Co? It is circled for you in the...
2. For the ELISA assay depicted below, what do the arrows indicate? Click here to enter answer 3. What would happen in terms of the colorimetric readout if you forgot to do the wash step after Step D (above)? Click here to enter answer-
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
5) The diagram below represents the energy diagram for the reaction of 1-butyl chloride with water. On the diagram, draw and indicate the position of each of the following: (0.9 points) a) structures of reactants, products, and intermediates (indicate the position of each on the diagram) b) indicate the positions of all transition states (label as "TS") c) indicate the activation energy of the rate-determining step (label as Ea) Energy Reaction Coordinate 6) Below is an energy diagram that represents...
Answer please?
The sequence below represents a middle section of the template strand of DNA of a structural gene in an eukaryote organism. Please fill in the blanks that correspond. The consensus sequences that the spliceosome recognizes are marked in red. The intron(s) are marked in lowercase. YOUR RESPONSES SHOULD ALL BE IN UPPER CASE. Amino acid sequences should be written in the format ALA-TYR-LEU Stop codon is not written. DNA: 3 CATGGACAGgtaagaatacaacacagGTCGGCATGACG 5' What would be the immature RNA...
The diagram below (the one on the right) represents the reaction
written above it (on top of the picture). Assign each step with a
reactant, a product or an intermediate. DRAW THE TRANSITION STATE
FOR EACH STEP.Keep in mind that it is easier to neutralize
charges than to create some. Nevermind what is handwritten on the
picture.
NOTE: THERE IS 2 STEPS (2 ARROWS) IN THE REACTION,
therefore there is 2 bumps on the diagram & 2 transition states
(that...
please help me with these questions. thank in
advance!
The diagram below represents an elevator used to lift concrete blocks to upper levels on a construction site. The belt travels at a velocity of 0.9 m/s and is inclined at 26° above the horizontal plane. Calculate the friction force and the coefficient of static friction between each block and the belt. Each block weights 16.5 kg. А NOT TO SCALE $300 Concrete is used in a variety of applications from...
1. (12 pts) Fill in the boxes over each arrow with the letter that corresponds to the reagents needed to perform each step of the multistep sequence shown below. Reagents may be used more than once, or not all. Pay close attention to the numbered carbons in the starting material, and be sure that your answer accounts for the position of these carbons in the final product. 1 3 3 3 2 2 MCPBA CF3SO3H A) HOCH3, DCC, DMAP E)...
D. The data below is for the reaction sequence A- Elementary step A, H/kJ mol A- B B- C C D B- C- E /kJ mol Hint: Use the data to prepare a graph of the reaction profile and then answer the questions below. Your graph should be properly labelled and drawn to scale. Potential Energy Reaction progress (a) Whichof the following steps has the smallest rate constant? AB Bc (b) Which of the following steps has the largest rate...
D) 16.94% Refer to the diagram below for questions 25 and 26: The Chart Below represents the Supply and Demand for Auto Insurance policies for teenagers with bad driving records. Price represents the annual premium paid for the policy to cover the student for a calendar year. The quantity represents the number of policies actually sold by the insurance companies to teenagers who want to drive. Price $1,900 $1,400 # 980- Demand -- puantity 25) 2500 3,800 6,500 If, in...
step by step please
Using the circuit diagram below, answer the following questions: Α Ω Β Ω 15.0 V (C Ω D Ω ma 15.0 v Assume the following resistor values for the resistors above: A-6 Ohms B-6 Ohms C-8 Ohms D-4 Ohms a) What is the value of l at resistor AQ? (12 points) b) What is the potential difference across the Ba? (4 points) What is the power at the Co? It is circled for you in the...
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