Answer: 7
Explanation: At a pH below their isoelectric point (pI) or net charge, proteins carry a net positive charge and conversely, At a pH above their pI they carry a net negative charge. as per the question protein carry a net negative charge. So the protein containing solution have to be 7 to become it neutral.
Question 7 1 pts An anion-exchange chromatography is used to purify a protein whose net charge...
2. (4 points) Multiple choice. Circle the best answer. In anion exchange chromatography, the charge on the column is We would expect a protein with ___ to bind most tightly to this column. a) positive, pH < PI b) positive , pH = pi c) positive , pH > pl d) negative , pH < PI e) negative , pH = PI f) negative , pH > PI
In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that can be used for ion exchange chromatography with this resin? any pH between 6.0 and 8.0 any pH above 6.0. any pH below 8.0 there is no pH range for this resin and protein X. Which of the following is...
Question 12 1 pts Which of the following technique is primary used to purify organic compounds? Thin layer chromatography O Reflux Distillation Recrystallization O Liquid-liquid extraction
need help w question Question 21 (1 point) Saved What is the charge on the solid phase of an anion exchange chromatography column? Depends on the salt used for elution O Negative Not enough information given O Positive Next Page Pago 21 min
Biochem help 2 14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
need help ASAP 3 pts Question 2 Acetaminophen is a medication used to treat pain and fever and contains Carbon, Hydrogen, Oxygen, and Nitrogen. Combustion analysis of a 15.0-mg sample of acetaminophen produced 8.10 mg of water. What is the percent by mass of Hydrogen in acetaminophen? 4.4% 12.0% 7.5% 3.0% 6.0% Question 4 1.5 pts AgCl, FeCO3. Hg Bry, and Pbly are compounds that are insoluble in water. Which compound would be more soluble in acidic solution? AgCl only...
c. Equal to 7.0 Question 2 1 pts A solution whose concentration of H3O+ is between 1.0x10-8 Mand 1.0x10-'M would have a pH: a. Lower than 7.0 b. Higher than 7.0 c. Equal to 7.0 D Question 3 1 pts Black tea has a nHof 42 while Denei haennu 7 Alhirhaft
BIOCHEMISTRY HELP ASAP PLEASE!!! Question 7 3 pts The transcription and production of a protein called interferon-beta (FNB) is regulated VF-Blevel by a transcription factor called IRF3 which binds to DNA as a dimer of two 30KD Mutation monomeric proteins. The DNA-binding site of RF3 contains the following amino acids. Nere Arg - Lys - lle - Thr. I create four mutants of IRF3 by changing one of the amino acids of AD the DNA-binding site to serine, and then...
This Quiz: 10 pts poss Question Help Find the general solution of the system whose augmented matrix is given below. 3 -2 6 -4 14 0 12 -8 280 choose the correct answer below, O A. O B. c. OD. - 3x2 The system has no solutlons. X, =3 X, =32-33 X2 = 2x3 X2 = - 2 X, is free Xg is free Xg =7 Xa is free ck to select your answer.