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In order to separate protein X using ion exchange chromatography, I am using a resin with...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pKa is 6.0). If I wanted to seperate protein X, whose pl is 3.0x what is the pH range of the buffer that can be used for ion exchange chromatography with this resin ? A. any pH between 3.0 and 6.0 B. there is no pH range for this resin and protein X C. any pH above 4.0 D. any pH below 6.0
For a process to occur non-spontaneously, click all that apply O Keq> o the enthalpy of the system must be a large positive value TAS should be more positive. the overall entropy of the universe is decreased AG is positive In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
(3 points) A Student uses cation exchange chromatography to separate Protein 1 pi9.5) and Protein 2 pl 45). The column is equilibrated using a 20m phosphate buffer, pH 6.0 and then the sample containing Protein 1 and Protein 2 is applied to the column. After the sample is applied to the column, the column is first washed with a low salt buffer. Subsequently, the column is washed with a high salt buffer. The elution profile is shown below. Clearly indicate...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm...
I performed an Ion-exchange chromatography to purify my Beta-galactosidase protein. If I observed a conductivity range of 10mS/cm to 16mS/cm what does that mean??
I want to separate a protein from a crude yeast extract by ion exchange at pH 7.4. Should I use a DEAE column or CM cellulose column? The isoelectric point (pI) of the protein is greater than the pH (pI = 9). Explain.
Question 89 Gluconeogenesis is the A) Result of amylase activity B) Formation of glycogen C) Formation of starches D) Formation of glucose from simple two and three-carbon precursors Question 90 The only known regulatory mechanism for pyruvate carbora A) Activation by acetyl Co4 B) Activation by phosphorylation C) Activation by CAMP D) Activation by NADH 20 5 Question 87 Isozymes are enzymes A) From one species that catalyze the same reaction B) From different species that catalyze the same reaction...
Based on the document below, 1. Describe the hypothesis Chaudhuri et al ids attempting to evaluate; in other words, what is the goal of this paper? Why is he writing it? 2. Does the data presented in the paper support the hypothesis stated in the introduction? Explain. 3.According to Chaudhuri, what is the potential role of thew alkaline phosphatase in the cleanup of industrial waste. CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
I need to write a conclusion for this lab. Based off these results what kinds of conclusions can be drawn? S OY Orre tes. A, B. Color, pH, Specific Gravity, and Electrolytes "Normal Urine Specimen" Test A. Color I clear "Abnormal Urine Specimen" slightly cloudy PH 2 1.10 gime! Specific gravity 0.70 glume - B. Electrolytes (Indicate absent - present +, strongly present ++) nornal Nat CI SO,2- PO,- 219 C-F. Glucose, Ketone Bodies, Protein, and Urobilinogen "Normal Urine Specimen"...