To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached...
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seperate protein X using ion exchange chromatography, I am using a
resin with carboxylates attached...
In order to separate protein X using ion exchange chromatography, I am using a resin with...
In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that can be used for ion exchange chromatography with this resin? any pH between 6.0 and 8.0 any pH above 6.0. any pH below 8.0 there is no pH range for this resin and protein X. Which of the following is...
For a process to occur non-spontaneously, click all that apply O Keq> o the enthalpy of...
For a process to occur non-spontaneously, click all that apply O Keq> o the enthalpy of the system must be a large positive value TAS should be more positive. the overall entropy of the universe is decreased AG is positive In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
(3 points) A Student uses cation exchange chromatography to separate Protein 1 pi9.5) and Protein 2...
(3 points) A Student uses cation exchange chromatography to separate Protein 1 pi9.5) and Protein 2 pl 45). The column is equilibrated using a 20m phosphate buffer, pH 6.0 and then the sample containing Protein 1 and Protein 2 is applied to the column. After the sample is applied to the column, the column is first washed with a low salt buffer. Subsequently, the column is washed with a high salt buffer. The elution profile is shown below. Clearly indicate...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm...
I performed an Ion-exchange chromatography to purify my Beta-galactosidase protein. If I observed a conductivity range...
I performed an Ion-exchange chromatography to purify my Beta-galactosidase protein. If I observed a conductivity range of 10mS/cm to 16mS/cm what does that mean??
chromatography mechanisms -In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract...
chromatography mechanisms
-In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP)...
7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP) from E. coli. Some physical and chemical properties of E. coli RNAP Molecular mass = 470,000 g/mol polypeptide composition (subunits): a (50 kDa), B (150 kDa) and 6 (70 kDa) pl = 5.34 substrates: NTPS cofactor: Mg Purification protocol. E. coli cells were broken using lysozyme, yielding a cellualar extract containing a proteome solution. 4M (NH4)2SO4 was added to the cellular extract. A white...
a-d plz 7. Protein purification. As a graduate student, the first protein I purified was RNA...
a-d plz
7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP) from E. coli- Some physical and chemical properties of Ecoli RNAP Molecular max470,000 g/mol polynentide compasitian (subunits): (50 kDa), k andok a pl = 5.34 substrates: NTPs cofactor: Mg? Purification protocol. E coli cells were broken usine Isozyme, yielding a cellualar extract containing a protcome solution 4M (NHOSO, was added to the cellular extract. A white protein precipitate was formed incuding RNAM...
Biochem help 2 14. What would be the net charge on the dipeptide Ser-His at pH-...
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that can be used for ion exchange chromatography with this resin? any pH between 6.0 and 8.0 any pH above 6.0. any pH below 8.0 there is no pH range for this resin and protein X. Which of the following is...
For a process to occur non-spontaneously, click all that apply O Keq> o the enthalpy of the system must be a large positive value TAS should be more positive. the overall entropy of the universe is decreased AG is positive In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
(3 points) A Student uses cation exchange chromatography to separate Protein 1 pi9.5) and Protein 2 pl 45). The column is equilibrated using a 20m phosphate buffer, pH 6.0 and then the sample containing Protein 1 and Protein 2 is applied to the column. After the sample is applied to the column, the column is first washed with a low salt buffer. Subsequently, the column is washed with a high salt buffer. The elution profile is shown below. Clearly indicate...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm...
chromatography mechanisms
-In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP) from E. coli. Some physical and chemical properties of E. coli RNAP Molecular mass = 470,000 g/mol polypeptide composition (subunits): a (50 kDa), B (150 kDa) and 6 (70 kDa) pl = 5.34 substrates: NTPS cofactor: Mg Purification protocol. E. coli cells were broken using lysozyme, yielding a cellualar extract containing a proteome solution. 4M (NH4)2SO4 was added to the cellular extract. A white...
a-d plz
7. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP) from E. coli- Some physical and chemical properties of Ecoli RNAP Molecular max470,000 g/mol polynentide compasitian (subunits): (50 kDa), k andok a pl = 5.34 substrates: NTPs cofactor: Mg? Purification protocol. E coli cells were broken usine Isozyme, yielding a cellualar extract containing a protcome solution 4M (NHOSO, was added to the cellular extract. A white protein precipitate was formed incuding RNAM...
Biochem help 2
14. What would be the net charge on the dipeptide Ser-His at pH- 6.04? (Choose the one best answer.) a) 1.5 b) +1; c) +0.5 d) 0 e) -0.5; 15. The pKa of a lysine side chain in a protein ending up on the outside of a globular protein has a different pKa than if the lysine is buried within the interior of a protein. What would be the expected pKa of a side chain of lysine...
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