Question

i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm high and 18 cm in diameter x 50 cm high. To keep resolution for the chromatograpy constant in this plant, which column should be used? (25 marks)

Answer 1

Chromatography ncale up :- scale by actor of up to asked we are 100. column diameter (d) = 20 cm conditions : 20cm bed height um particle size (dp) = 20 superficial velocity (u) = 30 cm/h be should used for neale up if column which the chromatography is to the resolution for be wnstant kept keeping resolution up ncale Am) yor have we constant, w

Gą, dez G, é, de -(0) where volume v= column G=Vg g change in concentration of gradient pen volume of Q : cle gradient Q : intet flow rate scale up, grader ten gradient un normally However not changed are particle sizi y G = G & dpiador :. eqn Q2 (2) als V2 V (2)

During scale up, bed height mobile phase Ov the name. kept linear is relocity constant column diameter changu. the :. only scaled given by 100) ( neal טטן Q₂ Q .: pom from (2) (2= length of wlumn) 2 22 Q. sls I (d₂²) kz 77 (d.?) , giveni 450cm L = 20 cm de ² x 50 100 di? 20 2 40 (e) d₂ ) d 140*d, (540)*2= 12.649 cm des 03

.. we will sileit the 14 cm 50 cm column . high diameter f).