I performed an Ion-exchange chromatography to purify my Beta-galactosidase protein. If I observed a conductivity range...
I performed an Ion-exchange chromatography to purify my Beta-galactosidase protein. If I observed a conductivity range of 10mS/cm to 16mS/cm what does that mean??
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I performed an Ion-exchange chromatography to purify my
Beta-galactosidase protein. If I observed a conductivity range...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pKa is 6.0). If I wanted to seperate protein X, whose pl is 3.0x what is the pH range of the buffer that can be used for ion exchange chromatography with this resin ? A. any pH between 3.0 and 6.0 B. there is no pH range for this resin and protein X C. any pH above 4.0 D. any pH below 6.0
In order to separate protein X using ion exchange chromatography, I am using a resin with...
In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that can be used for ion exchange chromatography with this resin? any pH between 6.0 and 8.0 any pH above 6.0. any pH below 8.0 there is no pH range for this resin and protein X. Which of the following is...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm...
chromatography mechanisms -In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract...
chromatography mechanisms
-In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
Question 7 1 pts An anion-exchange chromatography is used to purify a protein whose net charge...
Question 7 1 pts An anion-exchange chromatography is used to purify a protein whose net charge is zero when the solution pH is 8.0, what would be the pH of the protein containing solution have to be? Choose the best answer. O 12 0 7 8.9 10 6.0
Consider an important protein like beta-galactosidase, which breaks down lactose. Many bacterial species have beta-galactosidase. But...
Consider an important protein like beta-galactosidase, which breaks down lactose. Many bacterial species have beta-galactosidase. But that doesn't mean that the amino acid sequence of beta- galactosidase in all those bacterial species is exactly the same. Far from it. As bacteria diversified into different species over evolutionary time, beta-galactosidase encoded in the genomes of the bacteria also diversified. However, we can assume that even though beta-galactosidase diversified, it was under pressure to maintain structure and function, because the reaction it...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography....
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
I want to separate a protein from a crude yeast extract by ion exchange at pH...
I want to separate a protein from a crude yeast extract by ion exchange at pH 7.4. Should I use a DEAE column or CM cellulose column? The isoelectric point (pI) of the protein is greater than the pH (pI = 9). Explain.
4. (Challenge) You are purifying a protein from the brain of patients with an unusual type of...
4. (Challenge) You are purifying a protein from the brain of patients with an unusual type of dementia triggered by a coronavirus and you hypothesize that this protein may be a signature molecule of this type of dementia. To pursue this hypothesis, you collect brain tissue from patients post mortem, do differential centrifugation, density gradient centrifugation, CM (carboxymethyl cellulose) ion exchange chromatography and gel filtration. You then assess the purity of your protein using both native and SDS gel electrophoresis. The...
There are few questions I wanna ask about the Ion-exchange chromatography. 1. Why we measure the...
There are few questions I wanna ask about the Ion-exchange chromatography. 1. Why we measure the absorbance of the protein fraction at λ280 after purification by IEX? 2. After IEX, we are required to carry out BCA assay and L-DOPA assay to determine the protein's concentration and activity but why the instruction said I had to choose the fraction with highest absorbance value? 3. Can you think of one application of IEX to our daily life?
In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that can be used for ion exchange chromatography with this resin? any pH between 6.0 and 8.0 any pH above 6.0. any pH below 8.0 there is no pH range for this resin and protein X. Which of the following is...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm...
chromatography mechanisms
-In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
Question 7 1 pts An anion-exchange chromatography is used to purify a protein whose net charge is zero when the solution pH is 8.0, what would be the pH of the protein containing solution have to be? Choose the best answer. O 12 0 7 8.9 10 6.0
Consider an important protein like beta-galactosidase, which breaks down lactose. Many bacterial species have beta-galactosidase. But that doesn't mean that the amino acid sequence of beta- galactosidase in all those bacterial species is exactly the same. Far from it. As bacteria diversified into different species over evolutionary time, beta-galactosidase encoded in the genomes of the bacteria also diversified. However, we can assume that even though beta-galactosidase diversified, it was under pressure to maintain structure and function, because the reaction it...
SIRJE GEL OBJECTIVE: Partial purification of a protein, isocitrate dehydrogenase (IDH), using DEAE ion exchange chromatography. IDH will be eluted from the column using increasing concentrations of KCI. IDH will be tracked using the IDH activity assay and the extent of purification will be analyzed using SDS PAGE Last week we assayed for the activity of the enzyme, isocitrate dehydrogenase, from E.coli strains carrying a plasmid that over-expresses the E. coli IDH gene. To purify proteins from cel extracts, biochemists...
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