5) Describe one disadvantage to using microwave irradiation in the laboratory in terms of green chemistry...
these questions are in reference to gel filtration chromatography Fost lab questions on lab 8: Analysis of Laboratory Results 1. If the following mix of molecules were parified using sine exclusion chromatography, what would be the 6 WI onder in which the molecules pass chrough the opening in the bottom of the column? Mixture containingR hemoglobin, 65,000 daltons; myoglobin, 17,000 daltons; myosin, 180,000 daltons. rhe tw First molecule to appear Second molecule to appear Third molecule to appear 2. Ifa...
#2 is Q HiTrap column where it is anion exchange column not cation 2. Predict the order that the following proteins will elute from a CM Sephadex column run at pH 6.5 using a NaCI gradient: fibrinogen (pl-5.8), hemogloblin (pl7.1), ribonuclease A (pl7.8), lysozyme (pl-11), pepsin (pl-1.0) 3. In the laboratory we ran an immunoblot on fractions obtained from the alkaline phosphatase experiment. This procedure requires a number of steps, two different antibodies and a detection method. You should be...
5. One group of students in an int arguing that the pressu aroup of students in an introductory chemistry course is arguing that the pressures of a con- Chudrogen gas and a container of sulfur dioxide gas, under the following conditions, cannot equal. Both containers are sealed, with equal volume, at the same to that the pressures cannot be equal because a sulfur dioxide molecule has a much larger han diatomic hydrogen, and therefore exerts a much greater force when...
pre-laboratory Questions A 1 Explain why it is more efficient to make several extractions using small portions rather than one extraction using the same total volume. 2 Define the following terms: Drying agent intensity Drying agent capacity 3 Select the upper layer in each pair of mixed solvents (see Appendix C): Water and benzene Water and carbon tetrachloride Water and ether 4 Write an equation for the reaction of a benzoic acid with aqueous sodium bicarbonate. 5 Complete the following...
1.) Describe the goals of a Gel Filtration Chromotography Experiment??? 2.) Explain each key theoretical principle of a Gel Filtration Chromotography, and how they help acheive the goal???. 4.) Explain the key equations used in the Gel Filtration Chromotography experiment and the terms involved in the equation???? HI im trying to prepare for a lab/report and i have some questions i could use help with please :) over all having trouble seeing how everything ties together etc :) thank you...
Synthesis using Carbonyla-Substitution Chemistry Preamble This experiment involves the synthesis of target molecules using a-substitution chemistry. You are assigned two target molecules, one of which is best synthesized by an alkylation approach, while the other is best synthesized by an addition or condensation approach. My two target molecules are number 2 and number 12 in the last page. You must do the following: 1. Perform a retrosynthetic analysis on the two target molecules assigned to you. In each case the...
CHE115 Name: Tear this page out of your laboratory manual and submit it to your TA with your answers B. Post-Lab Assessment Questions: (25 pts) The post-lab assessment questions for each laboratory experiment are questions that you must complete at home and hand in to your laboratory instructor the following week These questions are designed to stimulate thought and may not always be simple to answer. You should always answer them as thoroughly as you are able to 1. (5...
what is the prediction of the unknown amino acids mixture based on the strip from thin layer chromatography sheet? The unknown mixtures can be ala plus asp, ala plus asp plus lys, lys plus asp or ala plus lys. anion exchange resin was used Tubes 2,3, 6 and 7 had a spot appear that was light pink We were unable to transcribe this imageWe were unable to transcribe this imageH-) to the column followed by a sufficient amount of the...
5. A student carries out a calorimetry experiment using HCl and NaOH. What effect will each of the following have on the calculated enthalpy of the reaction relative to the actual enthalpy of reaction? (larger, smaller or no change). Explain your reasoning a. The thermometer always registered a temperature 0.30 °C higher than the actual temperature b. The calorimeter constant used in the calculations by the student is larger than the actual value. (Hint: The best way to answer this...
Page 1 Synthesis using Carbonyl O-Substitution Chemistry Preamble This experiment involves the synthesis of target molecules using a-substitution chemistry. You are assigned two target molecules, one of which is best synthesized by an alkylation approach, while the other is best synthesized by an addition or condensation approach. You must do the following: 1. Perform a retrosynthetic analysis on the two target molecules assigned to you. In each case the organic starting material (what you have to work backwards toward) depends...