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A Lab has isolated a gene, CFE1, that encodes an enzyme (Cfe1) that completes one step of the conversion of caffeine into ade

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Answer #1

As per the question the cross of a mutant (named as Mutant 1) with the strain that has fully deleted CFE1 gene, after meiosis. results in 25% of spores that grow on the minimal media supplemented with caffeine.

This means 25% of the haploid spores from the cross can have the capacity to convert caffeine into adenine because they are surviving on the minimal media. This means one of the strain has the function copy of CFE1 gene that confers the growth of spores after the cross. As one stain has fully deleted the CFE1 gene.

This implies Mutant 1 has mutation in a gene other than CFE1.

So, the answer is D.

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