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Please explain the following question and answer all​​​

19. Plasmids designed for expressing a gene are different from plasmids for cloning A. harbor promoter and terminator sequencies flanking the multiple cloning site B. they use a selective marker other than ampicillin C. they do not require an origin of replication D. A and B 20. Milk yield in dairy cattle has a heritability of 60%, what does this mea n? A. 50% of the variation in yield is due to genes and 50% the environment, quantitative traits are not 100% genetic or 100% environmental B. Milk yield is due to many independent genetic loci each making a small contribution C. If a cow produces one more gallon of milk on average per week than the population mean about 0.4 gallons of the increased yield is due to genetics D. If a cow produces one more gallon of milk on average per week than the population mean about 0.6 gallons of the increased yield is due to genetics 21. Fill in the blanks. Li-Fraumeni syndrome is due to mutations in and results in A. BRCA1; high risk of skin cancer following UV exposure B. p53; high overall risk of developing cancer C. BRCA1; high risk of breast and ovarian cancer D. a promoter; high risk of colon cancer

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19)

ans -D ( A and B)

expression vector is used to introduce a specific gene into a target cell and commands the cell mechanism to produce gene product.it comprises of enhancers, promotor region,termination codon,transcription initaiation sequence and translation initiation sequence etc

20) ans - B- milk yied is due to many independent genetic loci each making a small contribution

21) ans -b - p53:high overall risk of developing cancer.

CHEK 2 AND TP 53 genes are involved in li -fraumeni syndrome where mutations occurs in p53 gene leading to cancers of soft tissue and breast cancer.

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