Homework Answers
Auxotroph is an mutant micro-organism that have defect in gene specifying a protien needed for biosynthesis of required nutrient i;e nutritional requirement is not shared by parent organism.
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What is an auxotroph? How were auxotroph's used to demonstrate one-gene coded for one enzyme. Explain...
What is the "one gene-one enzyme theory"? How was it developed (i.e. what experiments did Beadle...
What is the "one gene-one enzyme theory"? How was it developed (i.e. what experiments did Beadle and Tatum do)? Why do we now call it the "one gene-one polypeptide theory"? How does alternative splicing change this theory?
Explain how DNA methylation could be used to regulate gene expression in a tissue-specific way. When...
Explain how DNA methylation could be used to regulate gene expression in a tissue-specific way. When and where would de novo methylation occur, and when would demethylaiton occur? What would occur in the cells that give rise to eggs and sperm (germ-line cells)
What is the Central Dogma of genetics? -- EXPLAIN Corynebacterium diptheriae, the causative agent of diptheria,...
What is the Central Dogma of genetics? -- EXPLAIN Corynebacterium diptheriae, the causative agent of diptheria, secretes a toxin that enzymatically inactivates all molecules of elongation factor in a eukaryotic cell. What immediate and long-term effects does this have on the cellular metabolism of an infected person? -- EXPLAIN What is the difference between horizontal gene transfer and vertical gene transfer? -- EXPLAIN After running gel electrophoresis, what do the black bands represent? What can those bands tell us, and...
Background Information How can we predict where a coding gene will be in bacteria? And can...
Background Information How can we predict where a coding gene will be in bacteria? And can we then predict what protein will be produced? Take the DNA sequence below, for example. tcaggctttaattcatccgtgatctttgacgacggtaaatacgatgcagatataatacgatgaccgatgccaatcgaccgatcaaggaggcaccgaatggcgatgatggcgatgattgcgattaacgaagtggaacgcattatggcgggcattaacgaagatacccatgcgaccggcgaaaacgaaaccatttgcagctgcgcgaactttgaagaactgacccatgcgaccggccgcgaagcgacctaaaagtcgtaattacgtatcaagtcatgggccgcgggcgcccggcccactgactagactagggccgggcgcccgcggcccaccatataaataaaaaaaaaaaaaacgaggctatagctcatcaatgacct If you were a bacterial RNA polymerase, what sequence(s) should there be in this DNA for you to bind and begin transcribing? And if you found such sequence(s), where would you begin transcription? As a human being looking at this fragment of DNA, what type of consensus sequence(s)...
The gene machine program shows you what happens when lactose is present in E. coli, and...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
13. What are TWO of the insights Watson and Crick had with respect to the structure...
13. What are TWO of the insights Watson and Crick had with respect to the structure of DNA? a. DNA is a double-stranded helix held together by A:T or C:G base pairs b. DNA is a helix and has A:T or C:G base pairs c. DNA is a double-stranded helix and the amount of A=T and of G=C d. DNA has two strands in opposite orientation and the amount of A=T and of G=C 14. Griffith's experiment injecting a mixture...
how to show that? plz explain detail 5. In this problem, we will demonstrate that entropy always increases when there is a materal flow from a region of higher concentration to one of lower concen...
how to show that?
plz explain detail
5. In this problem, we will demonstrate that entropy always increases when there is a materal flow from a region of higher concentration to one of lower concentration. Consider a two- compartment system enclosed by rigid, impermeable, adiabatic walls, and let the two compartments be separated by a rigid, insulating, but permeable wall. We assume that the two compartments are in equilibrium but that they are not in equilibrium with each other. Then,...
genetic biology 5'-GCATGAGTCTGGTACGCTTTTAAAGC-3' 3-CATGCG-5' IIIII 3. (a) in the sequence above, what enzyme would you need...
genetic biology
5'-GCATGAGTCTGGTACGCTTTTAAAGC-3' 3-CATGCG-5' IIIII 3. (a) in the sequence above, what enzyme would you need to extend the short stretch of nucleotides shown on the bottom strand? (b) Write the sequence of the newly synthesized fragment and label its S' and 3' end. (c) The covalent bond between these adjacent nucleotides is what type of chemical bond? After using a chemical mutagen to generate mutations in a DNA sequence, scientists noted a mutation from C to T at the...
what is Agile? when, how, and why is ir used? explain in one page long if...
what is Agile? when, how, and why is ir used? explain in one page long if possible
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
The gene machine program shows you what happens when lactose is present in E. coli, and how the lac operon is under negative control. However, the lac operon is also under positive control from a protein called CRP, eAMP Receptor Protein. The absence of the lac repressor is essential but not sufficient for effective transcription of the lac operon. RNA polymerase also depends on the presence of CRP. Like the lac repressor, which can bind to the DNA and lactose....
13. What are TWO of the insights Watson and Crick had with respect to the structure of DNA? a. DNA is a double-stranded helix held together by A:T or C:G base pairs b. DNA is a helix and has A:T or C:G base pairs c. DNA is a double-stranded helix and the amount of A=T and of G=C d. DNA has two strands in opposite orientation and the amount of A=T and of G=C 14. Griffith's experiment injecting a mixture...
how to show that?
plz explain detail
5. In this problem, we will demonstrate that entropy always increases when there is a materal flow from a region of higher concentration to one of lower concentration. Consider a two- compartment system enclosed by rigid, impermeable, adiabatic walls, and let the two compartments be separated by a rigid, insulating, but permeable wall. We assume that the two compartments are in equilibrium but that they are not in equilibrium with each other. Then,...
genetic biology
5'-GCATGAGTCTGGTACGCTTTTAAAGC-3' 3-CATGCG-5' IIIII 3. (a) in the sequence above, what enzyme would you need to extend the short stretch of nucleotides shown on the bottom strand? (b) Write the sequence of the newly synthesized fragment and label its S' and 3' end. (c) The covalent bond between these adjacent nucleotides is what type of chemical bond? After using a chemical mutagen to generate mutations in a DNA sequence, scientists noted a mutation from C to T at the...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
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