Question

Following purification, the 6 x His tag can be removed from our protein of interest using Factor Xa protease. What is the utility of removing the 6 x His tag? Select one or more: The tag causes the target protein to bind to additional targets, creating artefacts. The detagged protein can be use for crystallographical or NMR studies where removal of the tag may be preferred The presence of a tag may interfere with the target's enzymatic activity The tag alters the subcellular localization of the target protein.

Answer 1

The correct option is [c]

It is because when 6 histidine tags are used as a marker, after purification process, it becomes necessary to remove them because it interferes with the downstream processes and enzymatic activity of the target protein.

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